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Suppressors of spindle checkpoint defect (such) mutants identify

By Janice Nelson,2014-11-26 13:38
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Suppressors of spindle checkpoint defect (such) mutants identify

    SUPPLEMENTAL TABLE 1 Duration from NEBD to NER in the suppressors of the mdf-1(gk2) lethal phenotype

    Embryo (genotypes) NEBD-NER(s) ? SE t-test t-test

    (n) Wild type F mdf-1(gk2) 2

    Wildtype (N2) 287.3 ? 7.0 (10) 1.0 0.067 F2 unc-46(e177) mdf-1(gk2) 304.1 ? 7.8 (10) 0.067 1.0

    Wildtype (N2) 16ºC 335.3 ? 7.8 (10) 0.0015* 0.047

    -8-8unc-46(e177) mdf-1(gk2); emb-30(tn377ts) 16ºC 651.3 ? 14.3 (10) 1.0X10* 5.0X10*

    -7-7emb-30(tn377ts) 16ºC 627.7 ? 22.2 (10) 2.4X10* 3.1X10*

    -9-8unc-46(e177) mdf-1(gk2); h1960 628.9 ? 16.0 (10) 7.4X10* 2.3X10*

    -7-7h1960 596.6 ? 10.7 (7) 5.3X10* 1.6X10*

    -7-7unc-46(e177) mdf-1(gk2); fzy-1(h1983) 543.3 ? 16.3 (10) 3.57X10* 9.0X10*

    -10-10fzy-1(h1983) 515.2 ? 3.1 (10) 3.19X10* 5.59X10*

    -5-4unc-46(e177) mdf-1(gk2); h1959ts 484.4 ? 24.8 (10) 3.8X10* 1.1X10*

    -3-3h1959ts 427.3? 13.8 (5) 5.0X10* 4.3X10*

    -6-6unc-46(e177) mdf-1(gk2) h1992 421.3 ? 6.7 (10) 1.1X10* 2.0X10*

    -5-4unc-46(e177) mdf-1(gk2); h1962ts 413.8 ? 13.2 (10) 1.5X10* 1.0X10*

    -4-3h1962ts 398.6 ? 18.7 (10) 2.5X10* 2.1X10*

    -6-5unc-46(e177) mdf-1(gk2); h1989 391.2 ? 9.4 (10) 7.0X10* 7.2X10*

    -4-3unc-46(e177) mdf-1(gk2); h1988ts 378.0 ? 13.0 (10) 1.5X10* 2.3X10*

    -4-4h1988ts 380.6 ? 10.2 (5) 2.5X10* 4.0X10* unc-46(e177) mdf-1(gk2) h2168 357.7 ? 14.7 (10) 0.0019* 0.021* unc-46(e177) mdf-1(gk2); h1987 324.7 ? 10.2 (10) 0.018* 0.071

    h1987 291.8 ? 8.5 (5) 0.70 0.67 unc-46(e177) mdf-1(gk2); h1958 309.6 ? 12.7 (10) 0.19 0.77

    h1958 296.6 ? 10.0 (5) 0.20 0.25 unc-46(e177) mdf-1(gk2) h1985 293.1 ? 5.3 (10) 0.29 0.22

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SUPPLEMENTAL FIGURE 1

Quantification of securin accumulation in such mutants

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    SUPPLEMENTAL FIGURE 2 Correlating mitotic delay with survivability (%fertile progeny) and genome stability (Him)

A B

    1060

    508

    406

    30

    4

    CIN (% males)20

    2Survivability (% fertile)10

    001.01.21.41.61.82.02.21.01.21.41.61.82.02.2Mitotic timing (fold increase)Mitotic timing (fold increase)

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