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Astragalus extract of anti-inflammatory, analgesic, anti-anoxia and anti-fatigue effects of_20

By Lynn Bailey,2014-10-30 10:37
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Astragalus extract of anti-inflammatory, analgesic, anti-anoxia and anti-fatigue effects of_20

Astragalus extract of anti-inflammatory, analgesic, anti-

    anoxia and anti-fatigue effects of

     Abstract Objective To investigate the extract of

    astragalus anti-inflammatory, analgesic, anti-anoxia and anti-

    fatigue effect. Methods 160 mice were randomly divided into four groups: control group, Astragalus large, medium and small-dose group, extract of astragalus way to ig

    administration, the control group fed an equal volume of saline. Use of xylene-induced mouse ear swelling method of the

    shell to measure the weight of ear pieces; with acetic acid-

    induced writhing frequency, determination of the number of writhing response in mice; used to record the atmospheric oxygen-resistant mice were resistant to hypoxia time; used colorimetric method for determination of swimming, 45 min after the mice liver glycogen and serum lactic acid content. The results of the xylene extract of astragalus auricular swelling-induced inhibition can reduce the acetic acid induced writhing number of times, extending the survival time under hypoxic conditions, mice 45 min after swimming glycogen of the original content of a marked increase in lactic acid content significantly decreased compared with the control group were significantly different. Conclusion Astragalus extract has a

    certain anti-inflammatory, analgesic, anti-anoxia and anti-

    fatigue effect.

     Key words extract of astragalus; anti-inflammatory;

    analgesic; hypoxia tolerance; anti-fatigue

     Astragalus has qi Sun, solid form antiperspirant, wound

    care and the benefits of water swelling of the myogenic effect, for the treatment of the table virtual spontaneous perspiration, Yang and edema of the blood off the blood numb embolism. In this experiment, the water extract of astragalus anti-inflammatory, analgesic, anti-anoxia and anti-fatigue

    effect of a preliminary study.

     1 Equipment

     1.1 Preparation of Astragalus bio-active ingredients

     Astragalus purchased in Yanji City, Jilin Province, large pharmacy health care by Yanbian University College of

    Pharmacy, Health and Medicine Teaching and Research was identified as the real thing. Astragalus boiling water three times, collecting decoction, filter, check fluid filtration, concentration, drying, very dry. 1 g of dry powder is

    equivalent to 4.5 g crude drug.

     1.2 lactic acid and liver glycogen reagent kit purchased from Nanjing Jiancheng Bio-Products Co., Ltd., and other

    reagents were of analytical grade.

     1.3 Animals

     Kunming mice, male and female in half, weighing 18 ~ 22

    g, from the Yanbian University School of Medicine Laboratory Animal Section.

     1.4 Instrument

     GB204 Precision Electronics, said, JCS-500 count

    electronic scales, 722 spectrophotometer, mice swimming pool, wide mouth bottles (250 ml).

     2 Methods

     2.1 The group taking 160 mice were randomly divided into four groups: control group and astragalus large, medium and small-dose group. Large, medium and small dose group respectively 0.5,0.25,0.125 g / kg dose of daily gavage, a

    continuous 7 d, control group fed an equal volume of saline.

     2.2 Anti-inflammatory experiment 10 mice in each group, the Last gavage 1 h after each uniformly coated mouse ear inflammation induced by xylene, 0.05 ml. 30 min later, mice

    were killed off neck, cut ear shell, with diameter of 8 mm hole puncher to take bilateral symmetry at the ear piece weighing, with its weight difference of swelling index.

     2.3 Analgesic experiment 10 mice in each group, the Last gavage 30 min later, mice in each group, respectively per 10 g body weight intraperitoneal injection of 6 g / L acetic acid 0.1 ml, observed within 15 min whether or writhing in mice response to the emergence of the performance of writhing with abdominal contraction, body distortion, hindlimb extension,

    and creeping lines and so on.

     2.4 hypoxia experiment 10 mice in each group, the Last gavage 24 h after the mice were placed one by one only of lime containing 20 g of sodium in 250 ml wide mouth bottle sealed, recording each survival time of mice.

     2.5 Anti-fatigue experiment 10 mice in each group, the

    Last gavage 24 h after the mice in each group were 37 ? in

    temperature in the swimming pool 40 cm deep, 45 min later, remove the mouse, picking the eye to take blood, through the colorimetric Analysis of serum lactate and liver glycogen

    content. Reposted elsewhere in the paper for free download http://

     2.6 Statistical analysis

     Data (? s) that the application of statistical software SPSS10.0 analysis of variance.

     3 Results

     3.1 Effects of astragalus on xylene-induced ear swelling

    of the shell

     Compared with the control group, large, medium and small doses of extract of astragalus can obviously against xylene-

    induced ear swelling effect (P <0.05 or P <0.01). The results in Table 1. Table 1, extract of astragalus on xylene-induced

    ear swelling effects (abbreviated)

     3.2 Effects of astragalus on acetic acid-induced writhing

    response of

     Compared with the control group, large, medium and small doses of extract of astragalus are able to significantly

    reduce the number of mice writhing (P <0.05 or P <0.01). The results in Table 2. Table 2 extract of astragalus on the glacial acetic acid-induced writhing number of effects

    (abbreviated)

     3.3 Effects of astragalus on the impact of experimental

    anti-anoxia in mice

     Compared with the control group, large, medium doses of astragalus extract can significantly prolong the survival time under anaerobic conditions (P <0.05), but the small doses there was no significant difference. The results in Table 3.

    Table 3 extract of astragalus in mice resistant to oxygen

effects (abbreviated)

     3.4 Effects of astragalus on the impact of anti-fatigue

    test

     Compared with the control group, large, medium-dose group

    after swimming in mice significantly increased liver glycogen content (P <0.05), low-dose group no significant change; and lactic acid content of drug-treated compared with control

    group decreased (P < 0.05). The results in Table 4. Table 4, extract of astragalus on mice liver glycogen and lactic acid

    content of (omitted)

     4 Discussion

     Astragalus Extract is rich in flavonoids. This study shows: Astragalus extract can significantly reduce the extent of ear swelling in mice to reduce the acetic acid stimuli in mice writhing number of results suggest that Astragalus extract has significant anti-inflammatory and analgesic

    effects, and to high-dose is more effective. Reported in the literature [1], flavonoids anti-inflammatory and analgesic

    mechanism of calcium antagonist on, its detailed mechanism

    needs further study.

     Fatigue is the body in certain environmental conditions, as long is too heavy, intense labor (including mental and physical) caused significantly reduced the efficiency of a temporary physiological and psychological phenomenon. The

    occurrence of fatigue is the result of multiple factors. Studies have found that the body's carbohydrate reserves are limited, high-intensity physical activity 1 ~ 2 h of fatigue may occur, accompanied by low blood sugar, muscle glycogen and

    liver glycogen depletion [2]. This study found that Astragalus

    can be at atmospheric pressure under hypoxic conditions, to extend the survival time of mice, and further observed that the extract of astragalus can significantly improve post-

    exercise blood lactate clearance rate, increased liver

    glycogen content, this may be Astragalus part of the mechanism

    of anti-fatigue effect, other mechanisms need to be further studied.

     References

     [1] Long Jinjiang. Rutin Research Situation and Prospect [J]. Chinese Journal of Traditional Chinese Medicine Information, 2002,9 (40): 39.

     [2] HOU Yue. Military Health Science [M]. Beijing: People's Medical Publishing House, 1998:384. Reposted

    elsewhere in the paper for free download http://

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