Astragalus extract of anti-inflammatory, analgesic, anti-
anoxia and anti-fatigue effects of
【Abstract】 Objective To investigate the extract of
astragalus anti-inflammatory, analgesic, anti-anoxia and anti-
fatigue effect. Methods 160 mice were randomly divided into four groups: control group, Astragalus large, medium and small-dose group, extract of astragalus way to ig
administration, the control group fed an equal volume of saline. Use of xylene-induced mouse ear swelling method of the
shell to measure the weight of ear pieces; with acetic acid-
induced writhing frequency, determination of the number of writhing response in mice; used to record the atmospheric oxygen-resistant mice were resistant to hypoxia time; used colorimetric method for determination of swimming, 45 min after the mice liver glycogen and serum lactic acid content. The results of the xylene extract of astragalus auricular swelling-induced inhibition can reduce the acetic acid induced writhing number of times, extending the survival time under hypoxic conditions, mice 45 min after swimming glycogen of the original content of a marked increase in lactic acid content significantly decreased compared with the control group were significantly different. Conclusion Astragalus extract has a
certain anti-inflammatory, analgesic, anti-anoxia and anti-
Key words extract of astragalus; anti-inflammatory;
analgesic; hypoxia tolerance; anti-fatigue
Astragalus has qi Sun, solid form antiperspirant, wound
care and the benefits of water swelling of the myogenic effect, for the treatment of the table virtual spontaneous perspiration, Yang and edema of the blood off the blood numb embolism. In this experiment, the water extract of astragalus anti-inflammatory, analgesic, anti-anoxia and anti-fatigue
effect of a preliminary study.
1.1 Preparation of Astragalus bio-active ingredients
Astragalus purchased in Yanji City, Jilin Province, large pharmacy health care by Yanbian University College of
Pharmacy, Health and Medicine Teaching and Research was identified as the real thing. Astragalus boiling water three times, collecting decoction, filter, check fluid filtration, concentration, drying, very dry. 1 g of dry powder is
equivalent to 4.5 g crude drug.
1.2 lactic acid and liver glycogen reagent kit purchased from Nanjing Jiancheng Bio-Products Co., Ltd., and other
reagents were of analytical grade.
Kunming mice, male and female in half, weighing 18 ~ 22
g, from the Yanbian University School of Medicine Laboratory Animal Section.
GB204 Precision Electronics, said, JCS-500 count
electronic scales, 722 spectrophotometer, mice swimming pool, wide mouth bottles (250 ml).
2.1 The group taking 160 mice were randomly divided into four groups: control group and astragalus large, medium and small-dose group. Large, medium and small dose group respectively 0.5,0.25,0.125 g / kg dose of daily gavage, a
continuous 7 d, control group fed an equal volume of saline.
2.2 Anti-inflammatory experiment 10 mice in each group, the Last gavage 1 h after each uniformly coated mouse ear inflammation induced by xylene, 0.05 ml. 30 min later, mice
were killed off neck, cut ear shell, with diameter of 8 mm hole puncher to take bilateral symmetry at the ear piece weighing, with its weight difference of swelling index.
2.3 Analgesic experiment 10 mice in each group, the Last gavage 30 min later, mice in each group, respectively per 10 g body weight intraperitoneal injection of 6 g / L acetic acid 0.1 ml, observed within 15 min whether or writhing in mice response to the emergence of the performance of writhing with abdominal contraction, body distortion, hindlimb extension,
and creeping lines and so on.
2.4 hypoxia experiment 10 mice in each group, the Last gavage 24 h after the mice were placed one by one only of lime containing 20 g of sodium in 250 ml wide mouth bottle sealed, recording each survival time of mice.
2.5 Anti-fatigue experiment 10 mice in each group, the
Last gavage 24 h after the mice in each group were 37 ? in
temperature in the swimming pool 40 cm deep, 45 min later, remove the mouse, picking the eye to take blood, through the colorimetric Analysis of serum lactate and liver glycogen
content. Reposted elsewhere in the paper for free download http://
2.6 Statistical analysis
Data (? s) that the application of statistical software SPSS10.0 analysis of variance.
3.1 Effects of astragalus on xylene-induced ear swelling
of the shell
Compared with the control group, large, medium and small doses of extract of astragalus can obviously against xylene-
induced ear swelling effect (P <0.05 or P <0.01). The results in Table 1. Table 1, extract of astragalus on xylene-induced
ear swelling effects (abbreviated)
3.2 Effects of astragalus on acetic acid-induced writhing