Astragalus from different habitats in the study of organochlorine pesticide residues in
【Abstract】 Objective To evaluate the different origin of the astragalus of organochlorine pesticide residues in medicinal quality evaluation. Methods water extraction
system, acetone, dichloromethane liquid-liquid distribution,
concentrated sulfuric acid sulfonation purification technology, flexible quartz capillary column (30 m × 0.32 mm
× 0.25 μm) SE 54, GC ECD detection of organochlorine
pesticide residues . Results The minimum detection limit is 0.1 ~ 0.5ng / g, the recovery was 72.6% ~ 114.8%; Gansu, Inner Mongolia and other main origin of organochlorine pesticide residues in Astragalus is far below the "Chinese Pharmacopoeia" standard. Conclusion A set of simple, workable detection of Radix Astragali organochlorine pesticide residues analysis.
Key words capillary gas chromatography; organochlorine pesticides; Astragalus; residues
Study on Residues of Organochlorine Pesticides in Radix
Astragali Samples from Different Field Origins
Abstract: ObjectiveTo program a quality evaluation of organochlorine pesticides residue on Radix Astragali produced from different places. MethodsSonication extraction system was used, in which acetone was the extractant. Based on salting
out principles, sodium chlorine was added to the extractant to separate the pesticides. Simultaneously, CH2Cl2 was also added to carry out liquid extraction, then it was purified by dense sulphuric acid, and finally detected by GC ECD. ResultsThe
detection limits were 0.1 ~ 0.5 ng / g, recoveries of organochlorine pesticides were 72.6% ~ 114.8% . Radix Astragali produced from Gansu, Neimeng ect were more lower than the standard of pharmacopeia.ConclusionA simple and
handling analytical method to detect the organochlorine pesticides of Radix Astragali has been established.
Key words: Capillary gas chromatography; Organochlorine pesticides; Radix Astragali; Residue
With the "return to nature" concept of the continuous deepening of the Chinese medicine industry has been greater opportunities for development. But one of the bottlenecks impeding the development of Chinese medicine is the cultivation of non-standard Chinese herbal medicine, heavy
metals, pesticide levels exceeded. The only way to solve this problem is to carry out the standardized GAP planting and management, of which one of the main elements of the establishment of pesticide residues in Chinese herbal medicine effective control method. In this study, the different origin of the astragalus by organochlorine pesticide residues testing, with a view to establish a set of simple, operable Radix Astragali organochlorine pesticide residues analysis.
An instrument and reagent
United States Varian cp3800 gas chromatograph, 63Ni ECD electron capture detector, 1177 injector; KQ 250E
Ultrasonic Cleaner; LDZ5 2 low self-balancing centrifuge;
ER 52AA rotary evaporator.
Radix Astragali by Tianjin Taiping Group of Inner
Mongolia Branch available; 666 (BHC) (α BHC, β BHC, γ
-BHC, δ BHC), DDT (DDT) (PP ' DDE, PP' DDD, OP '
DDT, PP' DDT), and PCNB (PCNB); were purchased from China, and biological products; other reagents were analytical pure.
2 method [1 ~ 3]
2.1 Preparation of reference substance solution
2.1.1 Preparation of reference substance stock solution precision that take 666 (BHC) (α