Astragalus Combination of Danshen on active ulcerative colitis patients with superoxide dismutase and malondialdehyde of

     【Abstract】 Objective To observe the astragalus, red sage root compatibility of ulcerative colitis (UC) in patients with

    superoxide dismutase (SOD) and lipid peroxidation product malondialdehyde (MDA) effects. Methods 36 cases of active UC patients, the application Astragalus, Salvia compatibility after 2 weeks of treatment were measured before and after treatment in patients with serum SOD and MDA levels, and with 30 cases of healthy people for comparison. The results of serum MDA in patients with UC were significantly higher; while SOD was significantly lower than control group; Astragalus, Salvia compatibility after treatment, serum MDA significantly lower than that before treatment, SOD were significantly higher than that before treatment. Conclusion Astragalus Salvia can reduce the compatibility of free radical levels in patients with UC, the UC has obvious effect.

     【Key Words】 Ulcerative colitis; superoxide dismutase; malondialdehyde; Astragalus; Salvia

     Ulcerative colitis (ulcerative colitis, UC) colonic mucosa and submucosa is characterized by inflammation of chronic inflammatory bowel disease, currently a lack of

    satisfactory methods of treatment are difficult to cure the World Health Organization as one of the modern. China's treatment of UC, the main drugs salicylic acid,

    corticosteroids, immune agents, etc., but more adverse

reactions [1]. Treatment of Chinese and Western medicine and

    therefore has become the main direction of UC therapy, clinical reports Astragalus Combination RSM UC achieved satisfactory results [2], but both combinations on the impact of oxygen free radicals in patients with few reports, we use

    Astragalus Combination Dan Participation in active UC patients treated, and in the treatment of patients with UC before and after the detection of SOD and MDA content in order to explore its therapeutic mechanism.

     1 Data and methods

     1.1 Data 2005 03 ~ 2006 09 patients with active UC

    were treated in 36 patients, aged 26 to 65 years, an average of 39 years of age; were male and 20 cases, female 16 cases; course of 2 months to 4 years, with an average duration of 15 months ; All cases were in line with 2001, Chengdu National Symposium on the development of inflammatory bowel disease activity of UC diagnostic criteria [3], based on clinical performance, colonoscopy, mucosal biopsy to diagnose, merging other diseases were not included in the study.

     The normal control group of 30 patients, aged 40 to 62 years, with an average 48-year-old, male 17, female 13 cases,

    both health checkup were. Case and control groups by age, sex no statistical significance.

     1.2 Methods

     1.2.1 Drugs for treatment of all cases of dexamethasone 5 ~ 12 mg / d, while application of Danshen injection 0.8 ~ 1.2 ml / kg (the equivalent of Pharmacognosy 1.5 g / ml), astragalus 0.6 ~ 1.0 ml / kg (equivalent to Pharmacognosy 2.0 g / ml), adding normal saline intravenous infusion, 14 d for a course of treatment, severe patients with 2 consecutive treatments. The condition of patients during treatment for

nutritional support and symptomatic treatment.

     1.2.2 observe the indicators and methods in all cases

    were within 24 h before treatment and treatment of 1 ~ 2 fasting venous blood samples after treatment were 3 ml, separation of serum placed in -20 ? refrigerator. Measured

    before treatment and treatment of 1 or 2 patients after treatment of serum superoxide dismutase (SOD) and lipid peroxidation product malondialdehyde (MDA) changes. MDA was determined by thiobarbituric acid (TBA) assay, SOD was determined by xanthine oxidase method kit were purchased from Nanjing Jiancheng Bio-engineering company, in strict

    accordance with kit instructions.

     In the treatment of 1 ~ 2 courses after colonoscopy and mucosal biopsy carried out to determine efficacy, strict reference to the National Symposium on Inflammatory Bowel Disease Clinical criteria developed by UC [3].

     1.2.3 Statistical Methods SAS8.0 statistical software, data, with ? s that was used to compare between the two groups were the number of F test was used to compare 22 q test.

     2 Results

     In addition to two cases of incomplete treatment, and the rest are all complete one or two courses.

     2.1 before and after treatment in patients with MDA, SOD changes in UC patients before MDA was significantly higher than the normal control group, SOD significantly decreased,

    the differences were significant (P <0.05). Astragalus Combination RSM 1 or 2 after treatment than before treatment MDA decreased significantly (P <0.01), compared with the control group, no significant difference (P> 0.05); SOD was significantly higher than that before treatment (P <0.01), compared with the control group, no significant difference (P> 0.05). The results in Table 1.

     Table 1 UC before and after treatment in patients with SOD, MDA content (abbreviated)

     With this group before treatment, ? P <0.01; with the

    normal control group, * P <0.05

     2.2 in 36 cases of clinical cure in 14 cases (41.1%), improved in 15 cases (44.1%), ineffective in 5 cases (14.7%), did not complete treatment in 2 cases, the total effective

    rate was 85.3%. Reposted elsewhere in the paper for free download http://

     3 Discussion

     OFR is a kind of high chemical reactivity of the oxygen-

    containing genes, tissues and cells produce oxidative damage, the body produces to defend against OFR damage the body has a set of equilibrium with its anti-oxidant systems, such as

    superoxide dismutase (SOD ) is a key enzyme in vivo elimination of OFR. Gastrointestinal mucosa is rich in OFR generation enzyme system, it has great potential for

    generating OFR [4,5]. UC study found that patients with OFR metabolite malondialdehyde (MDA) concentrations were significantly higher than the normal control group, while serum SOD activity was significantly lower than control group; and the other organs to exclude systemic disease cases, the lesions larger in scope than those lesions The smaller range of more significant [6]. Note inflammation of intestinal

    mucosa in UC patients with partial existence of OFR injury. Therefore, OFR injury is UC pathogenesis of ulcer formation, a

    possible course of protracted and even crucial factor, therefore speculated that anti-OFR is expected to be an

    effective treatment of UC.

     In recent years, the study found Salvia Astragalus Combination treatment of active UC patients have a certain effect, but its therapeutic mechanism involves very little. This study looked at patients with active UC before and after treatment Astragalus Combination Salvia changes in serum MDA, SOD, MDA concentration in serum of patients with UC was found

    significantly higher (P <0.05); while the SOD was significantly lower than the control group (P <0.05) ; Astragalus, Salvia compatibility after treatment, serum MDA significantly lower than that before treatment (P <0.01), SOD

    was significantly higher than that before treatment (P <0.01). The results of this study revealed the compatibility of Salvia Astragalus one of the mechanisms of effective treatment of UC is through scavenging oxygen free radicals completed.

     Chinese medicine practitioners believe that, UC pathogenesis of the disease of the spleen, heat and humidity for the onset of the standard, and blood stasis for the local pathological changes in the principles of its treatment spleen Qi, heat-dampness, cooling blood detoxification, blood

    circulation Huayu, Qu rot myogenic , Shibuya intestinal diarrhea. Radicals belong to the cult poison medicine category; astragalus has qi solid form, diuretic, care poison removing the abscess, gather sores myogenic the function, reduce UC serum MDA levels, elevated serum levels of SOD, indicating with the elimination of Astragalus inflammatory

    response, clear the OFR, the promotion of ulcer healing [7]; Salvia can expand blood vessels, reducing blood viscosity, scavenging oxygen free radicals [8]. Compatibility between the two conditions can be controlled to achieve a good therapeutic

    effect of time is much shorter, reducing the dose of glucocorticoids, reducing the possibility of complications, it is worth in clinical further.

     【References】

     [1] Ouyang Qin. To improve the research and diagnosis and treatment of inflammatory bowel disease [J]. Chinese Journal of Internal Medicine 2001,40 (1): 3.

     [2] Jian Yang, Yong-Mei Zhang. Dan participation in

    Astragalus treatment efficacy of 26 cases of ulcerative colitis [J]. China Coal Industry Medicine, 2005,8 (6): 600.

     [3] Chinese Medical Association of Gastroenterology will be. To regulate the recommendations of diagnosis and treatment of inflammatory bowel disease [J]. J Gastroenterol, 2001,6 (1): 56.

     [4] Ge He, Hong Sun. Oxygen free radicals and nitric

    oxide in the pathogenesis of ulcerative colitis the role of [J]. North China University (Natural Science Edition), 2003,4 (5): 411.

     [5] Ting Zhou, Lin Ping, Hui Pan, et al. Pathogenesis of ulcerative colitis and its research progress [J]. World J

Gastroenterol, 2003,11 (11): 1785.

     [6] HAO, Chang Xin-Ming, Wang public. Ulcerative colitis serum MDA and SOD changes in [J]. Gastroenterology and Hepatology, 2000,9 (2): 124.

     [7] Liu Jicheng, Chang Shan, YAN Chun-Hua, et al. Radix

    Astragali on hypoxic pulmonary hypertension in rat lung tissue superoxide dismutase and malondialdehyde of [J]. Yanbian University Medical Journal, 2002,25 ( 2): 97.

     [8] Huang Haibin, Li Lu, Chung-hua. Danshen injection on

    cerebral infarction in patients with serum LPO, SOD effects [J]. Zhejiang Medical Journal, 2002,26 (3): 47. Reposted elsewhere in the paper for free download http://