Artemisia annua L. and its preparation of volatile components analysis_597

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Artemisia annua L. and its preparation of volatile components analysis_597

    Artemisia annua L. and its preparation of volatile components analysis

     Abstract Objective To Artemisia annua L. and its

    preparations pairs of blue throat tablets volatile components analysis. Methods using gas chromatography mass

    spectrometry analysis identified, and with the normalized percentage content of its determination. The results of Artemisia annua L. volatile oil components were identified in 44, double-green throat piece 46 components were identified. Conclusion The method is stable and reliable for the analysis of volatile components of Chinese medicine.

     Key words Artemisia annua; pairs of blue throat plate; volatile components; gas chromatography mass spectrometry

     Abstract: ObjectiveTo analyse volatile constituents of

    Herba Artemisiae Annuae and its preparation (Shuangqing Yanhou tablet). MethodsThe constituents separated were identified by GC MS, and the percentage contents of compositions were determined by normalization method.Results44 components were

    separated and identified in volatile oil from Herba Artemisiae Annuae, and 46 components were separated and identified in Shuangqing Yanhou tablet.ConclusionThe method is stable, reliable and suitable for the volatile constituent analysis of traditional Chinese medicine.

     Key words: Artemisia annua L.; Shuangqing Yanhou tablet;

Volatile constituents; GC MS

     Artemisia annua, scientific name Artemisia annua Artemisia annua L., Asteraceae Artemisia for annual herb, with heat Jieshu, in addition to steaming, cut the effectiveness of

    malaria. Evil for the summer heat, yin deficiency fever, night fever as early as cold, Guzheng Tuberculosis fever, malaria, cold and heat, damp, jaundice card [1]. "Shen Nong's Herbal Classic" claim that "Wu Chuang attending scabies itching and

    scab, and kill the lice, and in administering to stay hot in the joints, the eyesight." "Japan-Zi" that its "leaves stems

    roots can be Buzhongyiqi, Qingshenjianfei fill labor." Pairs of blue throat piece according to the Department of Traditional Chinese Medicine theory group of parties, the use of modern pharmaceutical extraction process developed effective parts made of dual-use sublingual tablets.

    Functional aromatic thoroughly evil, heat detoxification, Sheng Jin Runzao, Liyan pain. Primarily for the treatment of

    acute and chronic pharyngitis, laryngitis, tonsillitis, oral mucosa ulcers. Artemisia annua L. volatile oil, one of its main effective site. Have been reported results show that different parts of Artemisia annua L. volatile oil components

    are quite different, in order to develop resources to provide the scientific basis for the use of Artemisia annua, and to explore the preparation process and compound combinations on the impact of volatile components, we used GC MS method

    middle region of Sichuan Luzhou Artemisia annua and its preparations Shuang-Qing throat tablets volatile components of the preliminary study.

     1 Equipment

     1.1 Instrument gas chromatograph, GC 17A (Shimadzu);

    gas chromatography mass spectrometer, QP5050 type

    (Shimadzu); CLASS 5000 workstations, NISTEI spectral


     1.2 Materials Artemisia annua (wild, collected from the outskirts of Luzhou City, Sichuan Province, dried, identified as Artemisia annua Artemisia annua L. Part of the dry ground);

    pairs of blue throat tablets (Luzhou Medical College Medicine Institute self-control, batch number 051,214); reagents were analytical pure.

     2 Methods

     2.1 Preparation of sample solution

     2.1.1 cut into small pieces of Artemisia annua Artemisia

    annua oil to take (3 ~ 4 cm), plus 8 times the amount of water, soak 3 h, heated distillation 3 h, the early collection of distillates, re-distilled 0.5 h, to collect volatile oil, plus a small amount of non - sodium sulfate dehydration was

    light yellow-green transparent oily liquid, with a special flavor Artemisia annua.

     2.1.2 double take pairs of blue-chip blue throat, the

    throat piece 20 (10.05 g), add water 100 ml, set volatile oil flask measuring device, the self-determination of the top, add

    water to make full-scale parts, and acetic acid in the

    determination of the tube B ester 2 ml, heated to boiling, extraction 1 h, collected ethyl acetate layer, add a small amount of anhydrous sodium sulfate dehydration, that is too.

     2.2 Gas chromatography - mass spectrometry conditions for elastic quartz capillary column: DB 1,30 m × 0.25 mm ×

    0.25 μm; programmed temperature: initial temperature of 40 ?, maintained 12 min, to 4 ? / 10 min rate of heating to

    70 ?, maintained 10 min, to 6 ? / 10 min rate of heating to

    230 ?. Carrier gas He, flow rate 1 ml / min; split ratio 30:1; pre-column pressure 49 kPa; injection port temperature 280 ?; ion source EI, electron energy 70 eV; ion source temperature: 200 ?; scanning mass range: 300 ~ 600 amu; scan

    interval 0.5 s; injection volume 1 μl. Reposted elsewhere in

    the paper for free download http://

     3 Results

     In the above-mentioned experimental conditions, the volatile oil of Artemisia annua and two blue-chip analysis of

    the throat, respectively, by their respective total ion chromatogram of the total ion chromatogram of each peak by MS mass spectrum obtained by scanning through MS Computer data systems retrieval, artificial spectrum analysis, according to the chromatographic peaks of the mass sliver diagram and documentation checking, check the MS data [2 ~ 5], and the base peak, mass charge ratio and relative abundance in areas such as visual respectively, when compared to confirm the various peaks to finalize Artemisia annua essential oil and

    two green throat film chemical composition, the results shown in Table 1 ~ 2.

     Table 1 Artemisia annua L. volatile oil GC-MS analysis

    results (omitted)

     We can see from Table 1 ~ 2: Artemisia annua oil, relatively high levels of the components were as follows: Artemisia ketone (45.82%), () camphor (12.90%),

    eucalyptol (7.92%), large roots myrcene D (4.28%), camphene (4.21%), caryophyllene (3.75%), and so on. Pairs of blue throat tablets, relatively high levels of component were:

    menthol (45.58%), artemisia ketone (20.33%), () camphor

    (8.08%), eucalyptol (6.75%), palmitic acid ( 2.89%), caryophyllene (2.18%), and so on.

     Table 2 pairs of blue throat tablets GC MS analysis

    results (omitted)

     4 Discussion

     Artemisia annua oil and dual-green throat tablets

    volatile components, a total of Camphene (Camphene), β

    pinene (β-Pinene), β-myrcene (β Myrcene), 2,6 - dimethyl

     3 , 7 diene 2 ol (3,7 Octadien 2 ol, 2,6

     dimethyl ), eucalyptol (Eucalyptol), artemisia ketone (Artemisia ketone), artemisia alcohol (Artemisia alcohol), (-)

    - camphor () Camphor, myrtle enol Myrtenol, caryophyllene Caryophyllene, large root myrcene D Germacrene D, caryophyllene oxide Caryophyllene oide the same as other 12

    components. In the first seven kinds of the highest content of the main components, the two are the same as Artemisia ketone ,(-)- camphor, cineole, caryophyllene, large root myrcene D 5 species, and the proportion of broadly similar

    levels, indicating Artemisia annua oil and its agents in the main composition is similar, but in its content in terms of fewer components, the two are quite different. This may be related to compound preparation containing menthol related, we have added natural menthol, in addition to the main active ingredient menthol, but also may bring a small amount of other volatile components. In addition, it may be in the preparation process of Artemisia annua oil heat, and with compound Chinese herbal medicinal ingredients react with the other result, suggesting that preparation process and compound combinations on volatile oil composition have an impact.

     Luzhou wild Artemisia annua L. volatile components mainly Artemisia ketone (45.82%), () camphor (12.90%),

    eucalyptol (6%), large root myrcene D (4.28%), camphene (4.21% ), caryophyllene (3.75%), farnesol (2.01%), caryophyllene oxide (1.84%), 2,6 2-methyl 1,5,7 -

    octatriene 3 - ol (1.81% ), Artemisia alcohol (1.71%),

    trans - long pinocarveol (1.28%), neroli (1.25%), etc., which ZHONG Yu-Rong et al [6] reported in Xiamen, Fujian, Artemisia annua essential oil production is similar, but the and PENG, etc. [7] reported in Artemisia annua produced in Sichuan are very different, especially in the content of Luzhou great

    Artemisia annua ketones, the original production of Artemisia

    annua in Sichuan have not been reported, but higher levels of Pang reports dragon brain, in this species of Artemisia annua oil was found. This may be due to a specific origin, different

    from the growth environment is different cause. In addition, we drying fresh Artemisia annua, and take a non-exposure, dry

    way, get dry Artemisia annua was yellow-green, with rich aroma

    of Artemisia annua, better preserved volatile active



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     [2] Cong Puzhu. Mass Spectrometry in Natural Organic Chemistry [M]. Beijing: Science Press, 1987:49.

     [3] Masada Y. Analysis of essential oils by gas chromatography and mass spectrometry [M]. New York: John wile and sons Inc. 1976:32.

     [4] Professional Committee of Chinese Mass Spectrometry Society of Organic. Spices MS Atlas [M]. Beijing: Science

Press, 1992:33.

     [5] Heller SR, Milne GWA.EPA / NIH mass spectral data Base [M]. Washington, US: Government Printing office, 1980:46.

     [6] ZHONG Yu-Rong, Cui Lin. Artemisia annua L. volatile

    oil chemical constituents [J]. Traditional Chinese Medicine Bulletin, 1983,8 (6): 31.

     [7] PENG, Guozhen De, Zhang Jing-Cheng, et al. Artemisia

    annua constituents of volatile oil [J]. Chinese herbal medicine, 1996,19 (9): 458. Reposted elsewhere in the paper

    for free download http://

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