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Rhubarb anthraquinone-induced diarrhea and its mechanism of preliminary study_294

By Joshua Stevens,2014-11-25 12:15
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Rhubarb anthraquinone-induced diarrhea and its mechanism of preliminary study_294

Rhubarb anthraquinone-induced diarrhea and its mechanism of

    preliminary study

     Abstract Objective To compare the combination of rhubarb anthraquinone, free anthraquinone-induced diarrhea and to

    explore the role of the strength of related mechanisms.

    Methods fed with a high concentration of milk powder solution to create a high nutritional environment, in this study based on the combination of rhubarb anthraquinones and free anthraquinone-induced diarrhea effects in mice; and the use of

    factorial design analysis of milk powder-induced diarrhea in

    these the role of the role, and also inspected the combination of rhubarb and free anthraquinone anthraquinones in vitro pepsin activity. The results of free rhubarb anthraquinones 500,1 000 mg / kg could stomach contents of mice and a significant increase in small intestine contents; rhubarb combination of anthraquinone 500,1 000 mg / kg also significantly increased the weight of the contents of the small intestine in mice, but the pairs of mice influence the

    weight of stomach contents of weak; the drug group of mice can be a significant increase in protein concentration within the intestines. Factorial design experiments showed that both free anthraquinone or a combination of rhubarb anthraquinones can

    be significantly increased in normal mice (without using milk powder) in the stomach, small intestine and the entire gastrointestinal tract contents and protein content, and can significantly reduce the mouse small intestine, colon Cl-

    concentration and increased the concentration of mouse colon K; milk powder itself also has the similar role of rhubarb anthraquinones, with combination can enhance the role of rhubarb anthraquinones. In vitro experiments showed that both free anthraquinone or a combination of rhubarb anthraquinones on the activity of pepsin had no significant effect. Conclusion Rhubarb and integration of free anthraquinone

    anthraquinone all have a significant effect of diarrhea; its mechanism may be by stimulating secretion of the

    gastrointestinal tract to increase the protein concentration within the gastrointestinal tract resulting volume of catharsis.

     Key words rhubarb with rhubarb anthraquinones free anthraquinones stomach intestine colon

     The Inducing Diarrhea Effects and its Mechanisms of

    Anthraquinon from Rheum palmatum L.

     TANG Da

     xuan, TAN Zheng

     huai, LIANG Yuan

     yuan, CHENG Lei, HUANG Li

     1.Sichuan Institute of Chinese Materia Medica, Chengdu 610041, China;

     2.Yunnan Dali College, Yunnan 671000, China

     Abstract: ObjectiveTo compare the efficiency and

    mechanisms of diarrhea induced by anthraquinon and glycosides of anthraquinone from Rheum Palmatum L. in mice. MethodsThe lapactic effects of anthraquinon and glycosides of anthraquinone from Rheum Palmatum L. were studied in the mice which were given high concentration of powdered milk solution to induced supernutrition, and the effects of these drugs on the activity of pepsin were measured in vitro. The effect of milk on the lapactic action of anthraquinon and glycosides of anthraquinone were analyzed using the factorial design.

    ResultsThe anthraquinone of Rheum palmatum L . 500,1 000 mg / kg significantly increased the remnant in the gastric and small intestine in mice, and the glycosides of anthraquinone from Rheum Palmatum L. 500,1 000 mg / kg also significantly

    increased remnant in small intestine in mice. All these drugs could increase the concentration of protein in the gut in mice. The results of the factorical design showed that the anthraquinone or glycosides of anthraquinone of Rheum palmatum

    L. were able to remarkably increase the remnant and protein concentration in stomach, small intestine and all of gastrointestinal tract, reduce the concentration of Cl-in

    small intestine and colon, increase the concentration of K in

    colon in normal mice. Milk had the similar effect and could enhance the action of anthraquinone of Rheum palmatum L. In vitro, anthraquinone or glycosides of anthraquinone from Rheum palmatum L. had no action on the activity of pepsin. ConclusionThese results indicated that antharquinone or

    glycosides of anthraquinone from Rheum palmatum L. can induce diarrhea in mice. These actions may due to that anthraquinone can increase the secretion of protein in gut .

     Key words: Anthraquinone from Rheum Palmatum L. (ARP); Glycosides of anthraquinone from Rheum Palmatum L. (GARP); Stomach; Small intestine; Colon

     Polygonum plants of rhubarb Rheum palmatum Rheum palmatum L. medicinal rhubarb Rheum offcinale Baill. And tanguticum Rheum tangguticum Maxim.ex Balf.'s Roots and rhizomes. Bitter,

    cold. The spleen, stomach, colon, liver, gallbladder through. A diarrhea attack plot, adjusting Sinochem food, Pinggan descending gas, gallbladder back yellow, heat detoxification, Xiehuo cooling blood, promoting blood circulation to stop

    bleeding, swelling and other benefits of water efficiency. Modern is widely used in the digestive system diseases, acute and chronic renal failure, bleeding disorders, acute infections, acute poisoning rescue, as well as prevention and treatment of diabetic nephropathy, etc. [1], indicating

    rhubarb in clinical uses. Rhubarb-induced diarrhea in the past

    that the main ingredient is its conjugated anthraquinone, its role mainly in the large intestine site that the mechanism of its role of combined anthraquinones stimulate the large

    intestine and the bowel wall muscle submucosal plexus within the significantly promote the transverse colon and descending colon peristalsis on [2]. Our previous study found that free anthraquinone of rhubarb in vitro the role of guinea pig colon

    was stronger than that of combined anthraquinone, also have reported in the literature that the rhubarb and the active ingredients anthraquinone can significantly inhibit trypsin and pancreatic lipase et al [3], then the role of rhubarb's

    diarrhea is due to inhibition of intestinal protease and lipase activity, reduce fat and protein absorption in the intestine produced hypertonic environment? The sky is the primary role of combined anthraquinone or anthraquinone sequestered? To explore these issues, we have adopted a high

    concentration of milk fed to create a highly nutritious solution environment, in this study based on the combination

of rhubarb and free anthraquinone Anthraquinone-induced

    diarrhea and its mechanism.

     1 Materials and methods

     1.1 The combination of drugs rhubarb anthraquinones (Glycosides of Anthraquinone from Rheum palmatum L., hereinafter referred to as GARP): rhubarb and then extracted by boiling with isolated and purified by macroporous resin and its rhubarb anthraquinone is mainly bound, its purity is 36.5% ; Rhubarb free anthraquinones (Anthraquinone from Rheum palmatum L., hereinafter referred to as ARP), the free anthraquinone content of 85.6% drug, the above experimental drug by the Institute of Medicine of Traditional Chinese

    Medicine, Sichuan Yi Jin Haibo Shi rooms available. Pro with distilled water before use, or 40% milk solution, the concentration of suspension preparation of the required reserve. Tongbianling capsules, 0.25 g / grain, Tonghua Wu Chang Baishan pharmaceutical production, batch number 20020601-0249, temporary paired with distilled water before use the required reserve levels. Erie full cream sweet milk powder, Inner Mongolia Yili Industrial Group Co., Ltd., batch number 8.71288 million. Pro dubbed with distilled water before use the required reserve levels.

     1.2 Reagent total protein (TP) test kit, lot number 0106091; triglyceride (TG) test kit, lot number 0106061, by Mike Technology Co., Ltd. in Sichuan Province to provide. HC-

    988 A standard solution, lot number 20060227; HC-988 B

    standard solution, lot number 20,041,129 by the invasive medical equipment, Shenzhen Airlines Limited. Pepsin, 1:3000, Sigma products.

     1.3 Animal SPF level Kunming mice, male and female in half, the production of qualified证号for SCXK (Sichuan 2005-

    19), Sichuan Institute of Experimental Animal Center of Chinese medicine.

     1.4 Instrument colorimeter, UV-730 Semi-automatic

    biochemical analyzer, Shimadzu produced; electrolyte analyzer HC9883, Shenzhen Airlines Co., Ltd. medical equipment, creating production capacity.

     1.5 Methods

     1.5.1 integration and free anthraquinone Anthraquinone-

    induced diarrhea in mice the role of the literature [3] with slight changes, take 20 ~ 22 g mice were 68 male and female in

    half, can not help but water fasting 24 h, the animals were randomly is divided into six groups, namely, milk group, milk powder combined anthraquinone 500 mg / kg, milk powder combined anthraquinone 1 000 mg / kg, milk free anthraquinone

    500 mg / kg, milk free anthraquinone 1 000 mg / kg, and milk positive for drug group, experiment were fed the same day, drugs or other liquid capacity of 40% milk powder (1.0 ml / rats), mice were sacrificed 50 min after drug, take the stomach, small intestine and colon that take the whole weight, and then were washed with 2ml saline stomach , small intestine and colon contents, using filter paper absorb moisture weighed (net weight of various organs), the organ's overall weight reduction of various organs, namely the contents of the net weight. Wash out the contents collected, centrifuged and the supernatant were measured by the total protein and small intestinal contents in the TG content.

     1.5.2 Factorial design of milk on the combination of rhubarb anthraquinones and free anthraquinones Effect of diarrhea take 20 ~ 25 g mice were 60 male and female in half, can not help but water fasting 24 h, the animals were randomly divided into 6 group, normal control group (distilled water), powdered milk group, combined with anthraquinone 1 000 mg / kg, free anthraquinone 1 000 mg / kg, milk powder combined anthraquinone 1 000 mg / kg, and milk free anthraquinones 1 000 mg / kg the experiment were fed the same day or the same volume of drug solution or 40% milk powder and other volume of distilled water (1.0 ml / rats), mice were sacrificed 30 min after drug, take out the stomach, small intestine and colon, said taking the whole weight, and then respectively 2 ml physiological saline wash stomach, small intestine and colon

    contents, using filter paper absorb moisture weighed (net weight of various organs), whole weight by weight shall be re-

    contents. Wash out the contents collected, centrifuged and were determined to take on the Qing Ye total protein, determination of the contents of the small intestine and colon contents of K, Na, Cl-content and the TG content of small

    intestinal contents.

     1.5.3 integration and free anthraquinone anthraquinones

in vitro effects of pepsin using Mett method [4] Preparation

    of protein tube, about 1 ~ 2 mm diameter capillary uniform thickness cut into 5 cm long, with an egg, remove the egg white, After sufficient shaking with gauze filtration, the role of the above-mentioned capillary filled with egg white

    using siphon (with no air bubbles), and then placed in 85 ?

    hot water convinces protein coagulation, and after cooling, remove the capillary tubes with wax at both ends of the protein closed, storage refrigerator in the back. Experiment,

    take 2% pepsin 0. 1 mol / L hydrochloric acid 5 ml, placed in disposable test tubes, each treatment group subjects were added to the liquid 100 μl (DMSO preparation), the normal

    group by adding dimethyl Sulfoxide 100 μl, after shaking,

    into the above-mentioned protein capillary 2 (first folded end to the solid wax seal), Seal, 37 ? incubated for 24 h,

    measured at both ends of a transparent part of the capillary length (cm), the two end of the length of the sum of the amount shall have been digested protein.

     1.6 Statistical analysis of measurement data with ? s,

    said, using analysis of variance and t test. Analysis of synergies used analysis of variance. Reposted elsewhere in the paper for free download http://

     2 Results

     2.1 The combination of rhubarb and free anthraquinone anthraquinone-induced diarrhea effects in mice can be seen from Table 1, rhubarb free anthraquinones 500,1 000 mg / kg and Tongbianling 1 000 mg / kg could stomach contents of mice and small intestine contents increased significantly with the

    milk powder have more significant difference between the control group (P

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