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Antimicrobial effect of different dosage forms Atractylodes Experimental Study_797

By Regina Howard,2014-10-30 10:21
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Antimicrobial effect of different dosage forms Atractylodes Experimental Study_797

    Antimicrobial effect of different dosage forms Atractylodes Experimental Study

     Abstract Objective To observe the different dosage forms of the herb to disinfect the role of antimicrobial agents. Ways to use the herb essential oil sprays, volatile oil from

    Atractylodes fumigation liquid, as well as the herb smoked disinfection agents on the room, and then placed in the room in different parts of the medium plate inoculated Staphylococcus aureus, Escherichia coli, Pseudomonas

    aeruginosa bacteriostasis rate determination. The results of three kinds of dosage forms of the herb doses of antimicrobial agents in a suitable under the condition of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa has a certain extent. Conclusion three kinds of dosage forms of the herb can be used for air disinfection bacteriostatic agent may be based on the actual situation to choose the most appropriate way to apply.

     Key words Atractylodes Staphylococcus aureus Escherichia coli Pseudomonas aeruginosa inhibitory rate

     Study on the Inhibitive Effect of Different Forms of Herbal Medicine Changzhu Disinfectant

     YU Li hua, YANG Yue chang, LI Xi lin, MIAO Zhu

     lei, WANG Cai hong, CHENG Sheng min

     1.Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China;

     2.Tianping Community Hospital of Xuhui District, Shanghai, 200031, China

     Abstract: ObjectiveTo observe the bacteria inhibitive effect of different forms of Changzhu Atractylodes chinensis

    (DC.) Koidz disinfectant. MethodsThe bacteria inhibitive rates against Staphylococcus aureus, Escherichia coli and Bacillus aeruginosus were determined, which were inoculated in media plates and placed in different parts of the room when the room was sterilized with Changzhu disinfectant, which were used in forms of spraying agent, perfume and fumigatum. ResultsStaphylococcus aureus, Escherichia coli and Bacillus aeruginosus could be inhibited by these forms of Changzhu disinfectant when given suitable dosage.ConclusionThese three forms of Changzhu disinfectant can be used in air sterilization according to practical conditions.

     Key words: Changzhu disinfectant; Staphylococcus aureus; Escherichia coli; Bacillus aeruginosus; Bacteria inhibitive rate

     Atractylodes for the Asteraceae genus Atractylodes Atractylodes chinensis Atractylodes chinensis (DC.) Koidz dry

    roots [1], is a clinical used blindly Chinese medicine, taken orally with spleen dampness, expelling wind, cold-dispelling,

    the efficacy of eyesight can be used to Abdominal Distension, diarrhea, edema, beriberi atrophy lame, rheumatism Bitong, cold cold, bird mesh night blindness embolism. Another herb can be used for air disinfection, Ming Li's in the "Compendium of Materia Medica" on the record of the herb "can In addition to Wu Qi, ancient and modern epidemic, and year-old Dan,

    people tend to burn the herb to ward off evil and gas, it is a disease of epidemic multi-purpose of the [2]." So At present

    the herb is also widely used in clinical out-patient rooms,

    wards, etc. of the air disinfection. However, traditional methods of disinfection on the herb is mainly through the

    burning, so that it contains bactericidal substances are distributed out to achieve sterilization purposes, many are inconvenient to use, and fire also have a certain degree of danger [3 ~ 5]. Therefore, this study attempts to use modern

    methods to extract the herb's volatile oil effective

    sterilization ingredient, processed into liquid sprays and fumigation, and then made an inspection of its antibacterial effects and compared in order to further improve the herb of the disinfection method provides vitro.

     1 Materials

     Herb, medicine company, purchased from Shanghai by Shanghai Traditional Chinese Medicine Teaching and Research Li Xilin, an associate professor of pharmacy students were identified as the North Atractylodes Atractylodes chinensis

    (DC.) Koidz; Tween -80, distilled water, 95% ethanol;

    bacteria: Staphylococcus aureus , Escherichia coli, Pseudomonas aeruginosa (Shanghai Traditional Chinese Medicine Teaching and Research Section of Microbiology, University subcultured saved).

     2 Methods

     2.1 Preparation of antibacterial herb medicinal herb to take appropriate, crushing, over 30 mesh sieve, in volatile oil extractor to extract volatile oil components made of different dosage forms of the herb antibacterial agents: the

    herb essential oil spray, take appropriate amount of volatile oil, plus a small amount of spit Wen 80 to help dissolve,

    dilute with sterile distilled water into a low, medium and high concentrations of the herb sprays, concentration per ml

    is equivalent to 0.075 g, 0.15 g, 0.3 g crude drug; herb volatile oil fumigation liquid, take appropriate amount of volatile oil , with 95% ethanol, diluted into low, medium and high concentrations of the herb fumigation liquid concentration per ml is equivalent to 0.75 g, 1.5 g, 3 g crude drug; an alternative amount of the herb medicine powder, just add a small amount of 95% ethanol, transferred into paste, smoked the herb was prepared by agents as a reference.

     2.2 Detection of bacteriostatic effect of different

    bacteria were collected from frozen Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa strains recovered bacteria increasing nutrient broth, gradient dilution, counting, so that the number of bacteria per milliliter of bacilli in the three × 103 is about the same amount bacilli

    were inoculated into the broth agar plate surface (per Min 0.1 ml).

     Will be inoculated with bacteria in petri dishes placed in the room flat level (2 m × 3 m × 2.5 mm) diagonal and the

    central two floors, each Department's release 3, and the other two vertical walls in the room diagonal (2 m height) with double-sided adhesive paste all three flat petri dishes inoculated with bacteria. Room temperature controlled at about 20 ?.

     Spray disinfection, taking different concentrations of spray 100 ml, evenly spray doses per cubic meter of space consumption is equivalent to 0.5 g, 1 g, 2 g crude drug. After 1 h flat out and get 37 ? incubator cultured 48 h, counting