Antimicrobial effect of different dosage forms Atractylodes Experimental Study
【Abstract】 Objective To observe the different dosage forms of the herb to disinfect the role of antimicrobial agents. Ways to use the herb essential oil sprays, volatile oil from
Atractylodes fumigation liquid, as well as the herb smoked disinfection agents on the room, and then placed in the room in different parts of the medium plate inoculated Staphylococcus aureus, Escherichia coli, Pseudomonas
aeruginosa bacteriostasis rate determination. The results of three kinds of dosage forms of the herb doses of antimicrobial agents in a suitable under the condition of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa has a certain extent. Conclusion three kinds of dosage forms of the herb can be used for air disinfection bacteriostatic agent may be based on the actual situation to choose the most appropriate way to apply.
Key words Atractylodes Staphylococcus aureus Escherichia coli Pseudomonas aeruginosa inhibitory rate
Study on the Inhibitive Effect of Different Forms of Herbal Medicine Changzhu Disinfectant
YU Li hua, YANG Yue chang, LI Xi lin, MIAO Zhu
lei, WANG Cai hong, CHENG Sheng min
1.Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China;
2.Tianping Community Hospital of Xuhui District, Shanghai, 200031, China
Abstract: ObjectiveTo observe the bacteria inhibitive effect of different forms of Changzhu 〔Atractylodes chinensis
(DC.) Koidz〕 disinfectant. MethodsThe bacteria inhibitive rates against Staphylococcus aureus, Escherichia coli and Bacillus aeruginosus were determined, which were inoculated in media plates and placed in different parts of the room when the room was sterilized with Changzhu disinfectant, which were used in forms of spraying agent, perfume and fumigatum. ResultsStaphylococcus aureus, Escherichia coli and Bacillus aeruginosus could be inhibited by these forms of Changzhu disinfectant when given suitable dosage.ConclusionThese three forms of Changzhu disinfectant can be used in air sterilization according to practical conditions.
Key words: Changzhu disinfectant; Staphylococcus aureus; Escherichia coli; Bacillus aeruginosus; Bacteria inhibitive rate
Atractylodes for the Asteraceae genus Atractylodes Atractylodes chinensis Atractylodes chinensis (DC.) Koidz dry
roots [1], is a clinical used blindly Chinese medicine, taken orally with spleen dampness, expelling wind, cold-dispelling,
the efficacy of eyesight can be used to Abdominal Distension, diarrhea, edema, beriberi atrophy lame, rheumatism Bitong, cold cold, bird mesh night blindness embolism. Another herb can be used for air disinfection, Ming Li's in the "Compendium of Materia Medica" on the record of the herb "can In addition to Wu Qi, ancient and modern epidemic, and year-old Dan,
people tend to burn the herb to ward off evil and gas, it is a disease of epidemic multi-purpose of the [2]." So At present
the herb is also widely used in clinical out-patient rooms,
wards, etc. of the air disinfection. However, traditional methods of disinfection on the herb is mainly through the
burning, so that it contains bactericidal substances are distributed out to achieve sterilization purposes, many are inconvenient to use, and fire also have a certain degree of danger [3 ~ 5]. Therefore, this study attempts to use modern
methods to extract the herb's volatile oil effective
sterilization ingredient, processed into liquid sprays and fumigation, and then made an inspection of its antibacterial effects and compared in order to further improve the herb of the disinfection method provides vitro.
1 Materials
Herb, medicine company, purchased from Shanghai by Shanghai Traditional Chinese Medicine Teaching and Research Li Xilin, an associate professor of pharmacy students were identified as the North Atractylodes Atractylodes chinensis
(DC.) Koidz; Tween -80, distilled water, 95% ethanol;
bacteria: Staphylococcus aureus , Escherichia coli, Pseudomonas aeruginosa (Shanghai Traditional Chinese Medicine Teaching and Research Section of Microbiology, University subcultured saved).
2 Methods
2.1 Preparation of antibacterial herb medicinal herb to take appropriate, crushing, over 30 mesh sieve, in volatile oil extractor to extract volatile oil components made of different dosage forms of the herb antibacterial agents: the
herb essential oil spray, take appropriate amount of volatile oil, plus a small amount of spit Wen 80 to help dissolve,
dilute with sterile distilled water into a low, medium and high concentrations of the herb sprays, concentration per ml
is equivalent to 0.075 g, 0.15 g, 0.3 g crude drug; herb volatile oil fumigation liquid, take appropriate amount of volatile oil , with 95% ethanol, diluted into low, medium and high concentrations of the herb fumigation liquid concentration per ml is equivalent to 0.75 g, 1.5 g, 3 g crude drug; an alternative amount of the herb medicine powder, just add a small amount of 95% ethanol, transferred into paste, smoked the herb was prepared by agents as a reference.
2.2 Detection of bacteriostatic effect of different
bacteria were collected from frozen Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa strains recovered bacteria increasing nutrient broth, gradient dilution, counting, so that the number of bacteria per milliliter of bacilli in the three × 103 is about the same amount bacilli
were inoculated into the broth agar plate surface (per Min 0.1 ml).
Will be inoculated with bacteria in petri dishes placed in the room flat level (2 m × 3 m × 2.5 mm) diagonal and the
central two floors, each Department's release 3, and the other two vertical walls in the room diagonal (2 m height) with double-sided adhesive paste all three flat petri dishes inoculated with bacteria. Room temperature controlled at about 20 ?.
Spray disinfection, taking different concentrations of spray 100 ml, evenly spray doses per cubic meter of space consumption is equivalent to 0.5 g, 1 g, 2 g crude drug. After 1 h flat out and get 37 ? incubator cultured 48 h, counting
colonies.
Volatile oil fumigation liquid disinfection, fumigation to take different concentrations of the herb liquid, electric heating to completely evaporate consumption per cubic meter of space dose equivalent to 0.5 g, 1 g, 2 g crude drug, 1 h after the removal plate, sending 37 ? incubator for 48 h, counting
colonies.
Smoked agent disinfection, taking the herb smoke agents, ignition, amount of drugs consumed per cubic meter of space is equivalent to 1 g crude drug, 1 h after burn out flat and get 37 ? incubator cultured 48 h, counting colonies.
The control group was not disinfected and train count op.
Using SPSS 8.0 software package variance test to compare the number of colonies in each group, and the difference with the control group was significant, and calculate the
percentage of bacteria.
Inhibitory percentage (%)=( the control group the average colony number plate - plate colonies disinfection group) /
control group, the average plate number of colonies × 100%.
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3 Results
Experimental results show that three kinds of dosage forms of the herb doses of antimicrobial agents in a suitable under the condition of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa have a certain extent. Overall,
in which the herb essential oil spray in the low, middle and high doses of the antibacterial effects of three kinds of bacteria, compared with the control group, have significant difference. Atractylodes bacteriostatic effect of volatile oil
suffocating liquid worse than the volatile oil sprays only at high concentrations compared with control group significant difference. Herb smoked agent also has some antibacterial effects, especially for more obvious inhibition of Staphylococcus aureus. In addition, the volatile oil from Atractylodes Atractylodes essential oil sprays and fumigation with liquid antibacterial power while increasing the concentration increased, but no statistical difference. Three kinds of dosage forms of the herb antibacterial disinfectant
results were shown in Table 1 ~ 3. Table 1 Antibacterial spray the percentage of volatile oil from Atractylodes (slightly) Table 2 fumigation liquid volatile oil from Atractylodes inhibitory percentage (abbreviated) Table 3 smoked the herb
doses inhibitory percentage (abbreviated)
4 Discussion
The herb is rich in resources and low price. Modern
research confirms that the herb in the main antibacterial ingredient contained in its volatile oil. Herb essential oil
by a series of sesquiterpenoid composition. Isolation and identification of 55 species have been elements. The main components are: α elemene, β ocimene, atractylon, Mau
surgery alcohol, β eucalyptus oil alcohol [6]. Therefore, these studies lay the herb of the dosage form a good basis for reform, in particular, to enable the development of liquid volatile oil fumigation possible. Herb essential oil non-toxic
non-irritating odor, non-corrosive, perfectly suited to the
circumstances under which someone can be used for continuous
dynamic air disinfection applications such as ward, buses, subways and other occasions, other than the current air disinfection method [7], such as ultraviolet disinfection, ozone, and some chemical disinfectants must be safe. Practical
use from the hospital ward experience, direct combustion of the herb of the sterilization method is simpler, does not require special equipment, but the use of fire with security risks, and burning herbs also produce large amounts of smoke
may irritate the airways, in the crowded occasions is not suitable for use. In contrast, aerosol sprays though not necessarily to fire, do not produce smoke, but the spray process will generate a large number of tiny droplets, the process of settlement may be contaminated with the patient and medical staff clothing, is not very convenient to use. Thus, these two formulations the use of antimicrobial agents subject to many factors. In contrast, volatile oil from Atractylodes Fumigation is not the first two liquid formulations of the
shortcomings, but it is not as good as the inhibitory effect of the spray. Therefore, in practical work, consider using the occasion should be an integrated, operational feasibility, convenience, and cost and safety and other factors, choose one
of the most appropriate forms. In the experiments we also found that using the same disinfectant spray the same conditions, the inhibitory values between different flat-panel
large fluctuations, which may be in the spraying of drugs or
drug volatile, it is difficult to achieve in the whole space to completely disinfect uniformly distributed, leading to the actual levels of exposure are different, in fact, this is the current practice of air disinfection in the frequently asked questions. In addition, the overall look at three kinds of dosage forms of the herb bacteriostatic agent belonging to Gram-positive bacteria Staphylococcus aureus bacteria is greater than the same percentage of Gram-negative bacteria
Escherichia coli and Pseudomonas aeruginosa, and its mechanism remains to be further studied . Therefore, in practical applications due consideration shall be distributed using the sites flora species.
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Beijing: Military Medical Science Press, 2002:44. Reposted elsewhere in the paper for free download http://