DOC

Antibacterial activity of the leaves of Liquidambar formosana_466

By Crystal Ruiz,2014-10-30 10:19
14 views 0
Antibacterial activity of the leaves of Liquidambar formosana_466

Antibacterial activity of the leaves of Liquidambar formosana

     Author: Chung, Wang Xiaoli MA Lian-lan

     Abstract Objective To investigate the in vitro

    antibacterial activity of the leaves of Liquidambar formosana.

    Methods agar diffusion method and in vitro leaves of Liquidambar successive dilution method and conditions for common pathogenic bacteria in vitro antibacterial activity and minimum inhibitory concentration (MIC). Results Liquidambar leaves against Escherichia coli, Candida albicans no

    antibacterial effect; sweet gum leaves against Staphylococcus aureus, Staphylococcus albus, Shigella flexneri, Salmonella typhi, Pseudomonas aeruginosa are have an antibacterial effect, inhibition zone diameter between 13 ~ 25 mm. Different

    methods of extraction liquid antibacterial effect of a significant difference, in which sweet gum leaves, decoction the best antibacterial activity against Staphylococcus aureus, Staphylococcus albus, Shigella flexneri, Salmonella typhi,

    Pseudomonas aeruginosa The minimum inhibitory concentration of bacteria were 0.5,1,0.5,0.5,0.25 g / ml. Conclusion Liquidambar leaves against Staphylococcus aureus, Staphylococcus albus, Shigella flexneri, Salmonella typhi, Pseudomonas aeruginosa has good antibacterial effect.

     Key words Liquidambar leaves; antibacterial activity; minimum inhibitory concentration

     Abstract: ObjectiveTo research antimicrobial activity of Liquidambar formosana Hance's leaf.MethodsBy methods of agar plate diffusions and tube continuous dilution, to measure antimicrobial activity and minimal inhibitory concentration (MIC) of Liquidambar formosana Hance's leaf against pathogen and conditioned pathogen.Results (1) Liquidambar formosana Hance's leaf had not antimicrobial activity on E.coli,

    S.albicans. (2) Liquidambar formosana Hance's leaf had antimicrobial activity against S.aureus, S.epidermidis, S.flexneri, Salmonella Typhi, P.aeruginosa, the diameter of bacteriostatic circle was between 13 and 25 millimetre. The

    antimicrobial activity was significant different in different extracted drug liquid, among which the antimicrobial activity of water extraction of Liquidambar formosana Hance's leaf was the best, the minimal inhibitory concentration against S.aureus S.epidermidis, S . flexneri, Salmonella Typhi, P.aeruginosa, is 0.5,1,0.5,0.5,0.25 g / ml,

    respectively.ConclusionLiquidambar formosana Hance's leaf has good antimicrobial activity against S.aureus, S.epidermidis, S.flexneri, Salmonella Typhi and P.aeruginosa.

     Key words: Liquidambar formosana Hance's leaf; Antimicrobial activity; Minimal inhibitory concentration

     With the extensive use of antibiotics, resistant strains have increased every year, at present Staphylococcus aureus strains resistant to penicillin G, as high as 90% [1], in particular, methicillin-resistant Staphylococcus aureus

    (methicillin-resistant Staphylococcus aureus, MRSA) has become the most common nosocomial infection pathogen [2]. Some might even have a multi-drug resistant bacteria such as E. coli

    pathogenic strains of about 40% of streptomycin, chloramphenicol, tetracycline, penicillin and other antibiotics resistance [3]; Pseudomonas aeruginosa Many antibiotics have a natural or acquired drug resistance [4], this has caused great difficulty in clinical treatment, how to effectively combat bacterial infections and control of bacterial drug resistance and the spread is a long, complex and arduous task, and thus to find and the development of

effective antibiotics is essential. In this paper, agar

    diffusion and serial dilution method in vitro antibacterial test and the minimum inhibitory concentration (MIC) determination to explore the sweet gum tree leaves and conditions for common pathogenic bacteria in vitro antibacterial activity. Are reported below.

     1 Materials and Methods Tu

     1.1 The source of Staphylococcus aureus strains (ATCC25923), Staphylococcus albus (ATCC12228), Escherichia coli (ATCC25922), Shigella flexneri (ATCC27891), Salmonella typhi (ATCC14028), Pseudomonas aeruginosa (ATCC27853) , purchased by the identification of biological products in Beijing. Candida albicans (ATCC14053), Peking University First Hospital Fungal Culture Collection Center (Beijing) to provide.

     1.2 Liquidambar leaves were collected from Jiangxi,

    Nanshan District, herbs time between 5 June.

     1.3 Preparation of liquid

     1.3.1 50% ethanol to take sweet gum leaves, decoction (crude drug) 40 g, washed, plus amount of 50% ethanol solution soaked 30 min, heated to 100 ? 30 min, filter. Dregs together

    with moderate 50% alcohol solution, heating 100 ? 30 min,

    filter. The two filtrate combined with the slow fire concentrated to 10 ml (per ml of liquid containing 4 g crude drug).

     1.3.2 decoction with distilled water instead of 50%

    ethanol solution were prepared according to the above-

    mentioned liquid.

     1.3.3 50% ethanol extract obtained sweet gum leaves (crude drug) 40 g, washed crushed, and soaked in distilled

water and 10 ml mixing 2h, filtered, and then through the

    filter bacteria filtration sterilization device, made of 4 g / ml of crude drug.

     1.3.4 water extract with distilled water instead of 50% ethanol solution were prepared according to the above-

    mentioned liquid.

     1.4 Methods

     1.4.1 Preparation of the Bacterium Staphylococcus aureus, Staphylococcus albus, Escherichia coli, Shigella flexneri, Salmonella typhi, Pseudomonas aeruginosa inoculated in ordinary agar plate, temperature box at 37 ? cultured 18 h,

    paired with sterile saline equivalent to 300 million per milliliter concentrations of bacteria (Staphylococcus aureus and Staphylococcus albus dubbed the equivalent of 900 million per milliliter of the bacteria concentration). To Candida albicans were inoculated on Sabouraud agar plate, warm box at

    37 ? cultured 48 h, paired with sterile saline 1 ×

    108CFU/ml.

     1.4.2 agar diffusion method using sterile cotton swab picks up bacilli in the ordinary agar plate (Candida albicans agar plate with the Sarbanes-Oxley) for uniform-intensive

    crossed until the bacilli dry, with external diameter 5 mm of play hole punch device, each conga liquid 200 μl, flat on the

    tank temperature 37 ? cultured 24 h (Candida albicans

    cultured 48 h) observations, measuring inhibition zone diameters. Inhibition zone diameter, taking the average of the two experimental results. Reposted elsewhere in the paper for free download http://

     1.4.3 test-tube serial dilution method [5] According to the inhibitory results of the experiment, select the

    susceptible ones sweet gum leaves, decoction of its determination of the minimum inhibitory concentration. Take trumpet tube 7, number. Step 1 of each tube add 1 ml broth. And then the first one plus 1 ml liquid, with the straw in a

blowing smoke several times, so that liquid mixing with the

    broth, remove 1 ml added in section 2, mixing all out in paragraph 1 ml plus 3, and so diluted to 6 disposable to 1 ml, No. 7 without any liquid used as control. Step 3 bacilli per tube plus 50 μl, mixing, temperature box at 37 ? cultured 24

    h. And then removed from each tube was inoculated into a normal part inoculated agar plate, and then further incubated 24 h, observe the results. No bacterial growth in order to dilute the concentration of the highest minimum inhibitory concentration for the liquid.

     2 Results

     2.1 Liquidambar formosana extract of the leaves of various Escherichia coli, Candida albicans no antibacterial effect.

     2.2 Liquidambar formosana leaves of various extracts against Staphylococcus aureus, Staphylococcus albus, Shigella flexneri, Salmonella typhi, Pseudomonas aeruginosa Jieyou antibacterial activity, inhibition zone diameter of 13 ~ 25 mm of the Inter. The results in Table 1. Different methods of extraction liquid antibacterial effect of a significant

    difference, in which sweet gum leaves, decoction best antibacterial effect, 50% ethanol solution followed by frying, 50% ethanol extract less.

     2.3 Liquidambar leaves decoction against Staphylococcus aureus, Staphylococcus albus, Shigella flexneri, Salmonella

    typhi, Pseudomonas aeruginosa the minimum inhibitory concentration (MIC), respectively 0.5,1,0.5,0.5 , 0.25 g / ml. The results in Table 2.

     Table 1 Liquidambar leaves several liquid antibacterial circle diameter (abbreviated)

     Table 2 The in vitro sweet gum leaves decoction of the minimum inhibitory concentration (abbreviated)

     - Had no bacterial growth; + indicated that a small amount of bacterial growth; + + that many more bacterial growth; + + + means that a lot growth of bacteria

     3 Discussion

     Hamamelidaceae Liquidambar leaves of plants Sweetgum Liquidambar formosana Hance leaves [6,7]. "Chinese Medicine Dictionary" records Liquidambar leaves are bleeding, cure boils, cure dysentery, cure post-partum human umbilical Wind

    wind and efficacy in children [7]. Chinese sweet gum leaves civil useful against rheumatism, cure heat stroke, cure dysentery; literature [8] reported that sweet gum leaves contain 10 kinds of inorganic elements in the human body and

    essential to treat diabetes and lower blood pressure; but sweet gum leaves in the antibacterial effect aspects of domestic and not been reported in the literature. In this study, Chinese sweet gum leaves, as the development of antibacterial drugs provided a reliable experimental basis.

     Experimental results show that the sweet gum leaves against Staphylococcus aureus, Staphylococcus albus, Shigella flexneri, Salmonella typhi, Pseudomonas aeruginosa are significant antibacterial effect. Different methods of liquid extraction are significant differences in the antimicrobial effects, including antibacterial effect Liquidambar leaves decoction best, 50% ethanol solution followed by frying, 50% ethanol extract poor, which may be related to different

    methods of extracting the medicine solution different from the active ingredients. Because crude extracts of traditional Chinese medicine contains a variety of chemical composition, composition and pharmacological action complex and influenced

    by many factors. This only went for sweet gum leaf crude

    extracts, the sweet gum leaves, the extraction and analysis of the active ingredient to be studied further.

     References

     [1] Lu source. Medical microbiology, 5th ed [M]. Beijing: People's Health Press, 2000:64.

     [2] Appelbaum PC. The emergence of vancomycin-

    intermediate and Vancomycin-resistant Staphylococcus attreusl

    [J]. Clin Microbiol Infect, 2006,3 (12) Suppl 1:16.

     [3] Wen Yumei. Modern Medical Microbiology [M]. Shanghai: Shanghai Medical University Press, 1999:205.

     [4] Li Hongxia, Li Cong-Rong, Jin-Ling Shi, et al.

    Hospital, in vitro resistance of Pseudomonas aeruginosa

    infection in dynamic observation [J]. China Hospital Infection Journal, 2002,12 (8): 621.

     [5] Li Ying Lin. Clinical examination manual [M]. Jilin: Jilin Science and Technology Press, 1987:606.

     [6] Chang Chen. Modern Practical Materia Medica (Chinese

    copies) [M]. Beijing: People's Health Press, 2000:404.

     [7] Jiangsu New Medical College. Dictionary of Chinese medicine, the book [M]. Shanghai: Shanghai People's Publishing House, 1977:1261.

     [8] Lan Huang, Xiao-Ping Song. Hainan Liquidambar in the

    human body essential Determination and analysis of inorganic elements [J]. Trace Elements and Health Research, 2001,18 (1): 45. Reposted elsewhere in the paper for free download http:/ /

Report this document

For any questions or suggestions please email
cust-service@docsford.com