Amaranthus spinosus particle compound long-term toxicity
【Abstract】 Objective To study the particles of compound Amaranthus spinosus long-term toxicity, provide a reference
for clinical safety of drugs. Methods according to the
original herbs 58.0,29.0,14.5 g / kg, the rats were continuously fed with medicinal extracts of the original six weeks, and withdrawal observed 2 weeks, rats were observed during the administration of the general performance and toxicity. The results of 6-week administration and withdrawal observed during the two weeks no toxic reactions and delayed toxicity. Conclusion The materials to be used according to the clinical dose, means of treatment application is safe.
Key words compound the long-term toxicity in rats Amaranthus
term Toxicity Study of Fufang Cixian Granules on Rats
gang, WU Yu
qiang, ZHEN Zuo
wen, ZHOU Zhi
1. Pharmaceutical Factory of Guangxi Traditional Chinese
Medical University, Nanning 530023, China;
2.Guangxi Institute for Drug Control, Nanning 530021, China
Abstract: ObjectiveTo study the long-term toxicity of
Fufang Cixian Granules on rats to provide some data for its clinical use. Methods80 rats were randomly divided into 4 groups, each group was given the extract of Fufang Cixian Granules intragastrically at doses of 0,58.0 , 29.0,14.5 g / kg for 6 weeks. The animals were observed for 2 weeks after medicine withdraw. Parameters were examined including general condition, bodyweight, blood cell, blood biochemistry, organ index, and histopathological exmination. ResultsNo toxic reaction was observed compared with control group. ConclusionFufang Cixian Granules should be safe in its
clinical use with the recommended dosage.
Key words: Fufang Cixian Granules; Rats; Long term toxicity
Amaranthus spinosus compound particles independently of Guangxi College of Pharmaceutical R & D of new drugs, with a heat dampness, cooling blood to stop bleeding, swelling and other effects Removing silt. Bet for the hot and humid, ?, ?
period of hemorrhoids. The product from Amaranthus spinosus, wind, astragalus, 37, Chinese angelica, rhubarb, Burnet so on. For the safety of clinical use of drugs, while providing a theoretical basis for clinical applications, the varieties of long-term toxicity studies. The results reported as follows.
Healthy Wistar rats were 80 body weight (120 ? 20) g,
male and female in half, from Guangxi Medical Research Institute of Animal rooms available (Qualified证号- Guangxi
Medical dynamic Zi No. 11001). Self-feeding with solid feed.
Laboratory temperature (22 ~ 26) ? (air conditioning),
relative humidity 60% ~ 75%.
The product of the original medicinal herbs extract per milliliter is equivalent to the original 2.14 g, provided by the Guangxi College of Pharmaceutical Factory, batch number 980,326. Experimental Shike Jia deionized water diluted into a
suitable concentration of liquid for the animal enema (IG) to vote medicinal.
Man Ziehl concentrated liquid (Tianjin Development Zone, Zhongshan High-tech Development and Research Institute, batch number 970901); HiCN reference solution (Tianjin Development Zone, Zhongshan Hi-Tech Development and Research Institute of production, batch number 980,123); total protein determination of biuret reagent ( Yuan-sheng of Taiwan-funded Biotechnology
Co., Ltd., batch number 290,097), alanine aminotransferase Kinetic Determination of reagents (Yuan-sheng of Taiwan-funded
Biotechnology Co., Ltd., batch number 223,026), aspartate aminotransferase Kinetic Determination of reagents (Taiwanese yuan Health and Bio-Technology Co., Ltd., batch number
210,056), Enzymatic determination of blood urea nitrogen urea reagent (Yuan-sheng of Taiwan-funded Biotechnology Co., Ltd.,
batch number 340,041), Kinetic determination of creatinine
picric acid reagent (Taiwanese yuan of shares of Biology
Science and Technology Co., Ltd., batch number 210,056); Enzymatic determination of total cholesterol reagent (Australia Hobsbawm Biological Engineering Co., Ltd., batch number 9908), Enzymatic determination of blood glucose reagent (Australia Hobsbawm Biological Engineering Co., Ltd., batch number S1110006).