Amaranthus spinosus particle compound long-term toxicity studies_298

By Catherine Matthews,2014-10-30 09:30
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Amaranthus spinosus particle compound long-term toxicity studies_298

Amaranthus spinosus particle compound long-term toxicity


     Abstract Objective To study the particles of compound Amaranthus spinosus long-term toxicity, provide a reference

    for clinical safety of drugs. Methods according to the

    original herbs 58.0,29.0,14.5 g / kg, the rats were continuously fed with medicinal extracts of the original six weeks, and withdrawal observed 2 weeks, rats were observed during the administration of the general performance and toxicity. The results of 6-week administration and withdrawal observed during the two weeks no toxic reactions and delayed toxicity. Conclusion The materials to be used according to the clinical dose, means of treatment application is safe.

     Key words compound the long-term toxicity in rats Amaranthus

    spinosus particles

     The Long

     term Toxicity Study of Fufang Cixian Granules on Rats

     DENG Jia

     gang, WU Yu

     qiang, ZHEN Zuo

     wen, ZHOU Zhi

     1. Pharmaceutical Factory of Guangxi Traditional Chinese

    Medical University, Nanning 530023, China;

     2.Guangxi Institute for Drug Control, Nanning 530021, China

     Abstract: ObjectiveTo study the long-term toxicity of

    Fufang Cixian Granules on rats to provide some data for its clinical use. Methods80 rats were randomly divided into 4 groups, each group was given the extract of Fufang Cixian Granules intragastrically at doses of 0,58.0 , 29.0,14.5 g / kg for 6 weeks. The animals were observed for 2 weeks after medicine withdraw. Parameters were examined including general condition, bodyweight, blood cell, blood biochemistry, organ index, and histopathological exmination. ResultsNo toxic reaction was observed compared with control group. ConclusionFufang Cixian Granules should be safe in its

    clinical use with the recommended dosage.

     Key words: Fufang Cixian Granules; Rats; Long term toxicity

     Amaranthus spinosus compound particles independently of Guangxi College of Pharmaceutical R & D of new drugs, with a heat dampness, cooling blood to stop bleeding, swelling and other effects Removing silt. Bet for the hot and humid, ?, ?

    period of hemorrhoids. The product from Amaranthus spinosus, wind, astragalus, 37, Chinese angelica, rhubarb, Burnet so on. For the safety of clinical use of drugs, while providing a theoretical basis for clinical applications, the varieties of long-term toxicity studies. The results reported as follows.

     1 Equipment

     1.1 Animals

     Healthy Wistar rats were 80 body weight (120 ? 20) g,

    male and female in half, from Guangxi Medical Research Institute of Animal rooms available (Qualified证号- Guangxi

    Medical dynamic Zi No. 11001). Self-feeding with solid feed.

    Laboratory temperature (22 ~ 26) ? (air conditioning),

    relative humidity 60% ~ 75%.

     1.2 Drugs

     The product of the original medicinal herbs extract per milliliter is equivalent to the original 2.14 g, provided by the Guangxi College of Pharmaceutical Factory, batch number 980,326. Experimental Shike Jia deionized water diluted into a

    suitable concentration of liquid for the animal enema (IG) to vote medicinal.

     1.3 Reagents

     Man Ziehl concentrated liquid (Tianjin Development Zone, Zhongshan High-tech Development and Research Institute, batch number 970901); HiCN reference solution (Tianjin Development Zone, Zhongshan Hi-Tech Development and Research Institute of production, batch number 980,123); total protein determination of biuret reagent ( Yuan-sheng of Taiwan-funded Biotechnology

    Co., Ltd., batch number 290,097), alanine aminotransferase Kinetic Determination of reagents (Yuan-sheng of Taiwan-funded

    Biotechnology Co., Ltd., batch number 223,026), aspartate aminotransferase Kinetic Determination of reagents (Taiwanese yuan Health and Bio-Technology Co., Ltd., batch number

    210,056), Enzymatic determination of blood urea nitrogen urea reagent (Yuan-sheng of Taiwan-funded Biotechnology Co., Ltd.,

    batch number 340,041), Kinetic determination of creatinine

picric acid reagent (Taiwanese yuan of shares of Biology

    Science and Technology Co., Ltd., batch number 210,056); Enzymatic determination of total cholesterol reagent (Australia Hobsbawm Biological Engineering Co., Ltd., batch number 9908), Enzymatic determination of blood glucose reagent (Australia Hobsbawm Biological Engineering Co., Ltd., batch number S1110006).