Alpine Rhodiola on acetaminophen-induced acute liver injury in

    rats

     【Abstract】 Objective To observe the Rhodiola (Rhodiola sachalinesis A. Bor) Right, right, acetaminophen (Acetaminophen.AAP)-induced acute liver injury in rats.

    Methods Rhodiola Rhodiola extract or water extract of 1 g / kg to mice gavage 1 h after the irrigation 1 g / kg of acetaminophen, the changes in mortality of mice; advance 1 h with the different doses of Rhodiola Rhodiola extract or water extract treatment, reperfusion 0.5 g / kg of acetaminophen, to serum aspartate aminotransferase (AST / GOT), alanine amino -

    Transferase (ALT/GPT), Observation and the ethanol extract of Rhodiola Rhodiola rosea L. extract on liver injury in rats [1]. The results to mice to acetaminophen 1 g / kg gavage

    group, 24 h caused 80% of the mice after death; advance 1 h with the ethanol extract of Rhodiola rosea and Rhodiola rosea L. extract fed mortality rate respectively down to 30% and 20%, but also varying degrees of decrease in serum AST, ALT

    levels, and silymarin was no significant difference. Conclusion Rhodiola Rhodiola extract and water extract of acetaminophen-induced acute liver injury have some protective effect.

     Key words Rhodiola acetaminophen liver damage silymarin

     The Protective Effect of Rhodiola sachalinesis (A. Bor) against Acute Liver Injury Induced by Acetaminophen (AAP) in Mice

     Abstract: ObjectiveTo investigate the protective effect of Rhodiola sachalinensis (A. Bor) agaigt acute liver injury

    in mice induced by Acetaminophen (AAP). Methods Administered the mice with 1 g / kg ethanol or water extraction of Rhodiola sachalinensis, 1 h later, administered the mice with 1 g / kg ace taminophen (AAP), then observe the death rate.Administered the mice with different doses of ethanol or water extract of Rhodiola sachalinensis, 1 h later, administered the mice with the 0.5 g / kg Acetaminophen (AAP ), then determinea the aspartate aminotransferase (AST) and alanine aminotransferase (ALT). Results80% of the group administered with 1 g / kg acetaminophen (AAP) were dead 24 h later, and death rate of the group that were administered with Rhodiola sachalinensis ethanol extraction or Rhodiola sachalinensis water extraction 1h in advance declined to 30% and 20%, meanwhile the serum

    enzymes of AST, ALT were reduced, with the same effect as Silymarin. ConclusionRhodiola sachalinensis (A. Bor) have certain protective effect against liver injury caused by acetaminophen (AAP).

     Key words: Rhodiola sachalinensis (A. Bor); Acetaminophen

    (AAP); Silymarin; Liver injury

     Rhodiola sachalinensis as a perennial herb or sub-shrub

    vegetation, all herbal medicine, there are Qingfei cough, nourishing strong, and reinforce the role of the righting. In recent years, at home and abroad Rhodiola chemical

    composition, pharmacological effects and clinical application of the system carried out a more in-depth study. However, the

    role of a rare liver injury reported in the literature. Experimental observation of this high mountain Rhodiola on

    acetaminophen-induced acute liver injury in rats.

     1 Materials

     1.1 Animal Health male Kunming mice, weighing 20 ~ 22 g, from the Department of Yanbian University Medical Experimental Animal Section.

     1.2 herbs Rhodiola sachalinensis were purchased from large pharmacy drugs Yanji City, Jilin Province, was identified as authentic.

     1.3 Reagent Silymarin purchased from the Chinese biological products, batch number 050,603; acetaminophen (Acetaminophen.AAP) Guangzhou Baiyun Mountain Pharmaceutical Co., Ltd., lot number 0306021; aspartate aminotransferase (AST / GOT), alanine aminotransferase (ALT / GPT) kit were purchased from Nanjing Jiancheng Bio-Engineering Institute.

     1.4 Instrument Sp-4430 automatic biochemical analyzer,

    Japan Arkray companies; BS224-type electronic balance, Beijing

    Sartorius Instrument Systems, Inc.; SIGMA95308 automatic centrifuge, Sigma Corporation.

     2 Methods and Results

     2.1 Alpine Rhodiola on acetaminophen-induced mortality of

    the 30 mice were randomly divided into control group, mountain Rhodiola extract group and the water extract of Rhodiola sachalinensis groups of 10 . The control group fed with an equal volume of saline, ethanol extract of Rhodiola sachalinensis group with high mountains Rhodiola extract (0.3 g / kg) gavage, water extract of Rhodiola sachalinensis group with aqueous extract of Rhodiola sachalinensis (0.3 g / kg) gavage, 1 h after all the mice with acetaminophen (1 g / kg) gavage, 24 h after the circumstances of the death records of each group [2].

     2.2 Alpine Rhodiola on acetaminophen induced liver injury in the 90 mice were randomly divided into nine groups, namely control group, model group, control group, silymarin, alpine Rhodiola extract low-dose group, mountain Rhodiola extract in the dose group, mountain Rhodiola extract high-dose group, the

    water extract of Rhodiola sachalinensis low-dose group, the

    water extract of Rhodiola sachalinensis dose group, the water

extract of Rhodiola sachalinensis high-dose group , n = 10.

    Free two days after eating experiment, fasted before the experiment 16 h. Experimental treatment: control group and model group with an equal volume of saline gavage, Silymarin Silymarin water control group (0.2 g / kg) gavage, the

    experimental group with the ethanol extract of Rhodiola sachalinensis (0.1,0.3,0.5 g / kg ) and the water extract of Rhodiola sachalinensis (0.1,0.3,0.5 g / kg) gavage, continuous 4 d administration of each interval of 24 h, the last administration 1 h, the control group with 1% carboxymethyl-

    fiber Sodium (0.15 g / kg) gavage, the other groups were using acetaminophen solution (0.5 g / kg) gavage, 24 h mice were sacrificed after the removal of cervical [3]. Reposted elsewhere in the paper for free download http://

     2.3 Preparation of mouse serum carotid artery blood, put it aside, 3 000 r / min centrifugation 15 min, separating serum, home refrigerators ( 20 ?) in the cryopreservation,

    spare.

     2.4 Determination of indicators and methods of [4] learn

    from the separation of serum, in the Sigma95308 automatic biochemical analyzer was determined by aspartate aminotransferase (AST / GOT), alanine aminotransferase (ALT / GPT) activity.

     2.5 Statistical analysis of experimental data using

    statistical software SPSS8.0 analysis of variance, using ? s

    said that the difference between the groups using t test.

     Fatality rate of 2.6 pairs of mice were fed a one-time in

    order to acetaminophen (1 g / kg), 24 h control group after

    the death of eight, the mortality rate of 80%; Alpine Rhodiola extract group killed 3 ( 30%) and water extract of Rhodiola sachalinensis group killed two (20%). The results showed that ethanol extract of Rhodiola sachalinensis and Rhodiola sachalinensis extract on large doses of acetaminophen-induced

    death of a very significant protective effect of aqueous extract of Rhodiola sachalinensis was stronger than the role of the mountain Rhodiola extracts [5].

     2.7 Alpine Rhodiola extract the protective effect on liver injury in alcohol extract of Rhodiola sachalinensis acetaminophen-induced serum ALT, AST levels studied. The results in Table 1. Table 1 alcohol extract of Rhodiola sachalinensis in mice serum ALT, AST levels of impact

    (omitted)

     These results suggest that, given acetaminophen, the model group of AST, ALT levels were significantly increased compared with the control group significant difference (P