Allicin bladder cancer in mice in vivo and in vitro anti-tumor effects of experimental study_442

By Linda Weaver,2014-10-30 09:23
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Allicin bladder cancer in mice in vivo and in vitro anti-tumor effects of experimental study_442

Allicin bladder cancer in mice in vivo and in vitro anti-tumor

    effects of experimental study

     Abstract Objective To understand the allicin on mouse bladder tumor anti-tumor effect and mechanisms. Methods The MTT test of allicin directly assessed the cytotoxic activity, animal experiments showed that allicin has significant in vivo anti-tumor effect, and measured using LDH release assay cytotoxicity. The results of allicin directly to the bladder tumor cytotoxicity. High-dose treatment group compared with

    control group, tumor growth rate was obviously inhibited (P <0.01), after treatment of allicin produced lymphocytes against B16 tumor cells. Conclusion of allicin on bladder cancer has obvious anti-tumor effect. This effect may be

    related to direct cytotoxicity and immune responses. Allicin may be an effective treatment for superficial bladder cancer cavity.

     Key words garlic; anti-tumor; bladder cancer

     The Experimental Research on the Antitumor Effect of Allicin on Murine Bladder

     Abstract: ObjectiveTo understand anti tumor effect and

    mechanism of allicin on murine bladder.MethodsThe direct inhibition effects of allicin in vitro was evaluated by MTT Assay.Anti tumor effect of allicin in vivro was determined by animal experiment. CTL activity of lymphocyte was measured

    by the release of endogenous lactate dehydrogenase (LDH). Results Allicin had significant direct cytotoxicity to bladder cancer cells. In high dose group, it showed a marked delay in the appearance and growth of tumors after sc injection

    compared with control group (P <0.01 ). The body generated the cytotoxic lymphocyte responses to B16 tumor cells after treated with allicin.ConclusionAllicin has a marked anti

    tumor effect on bladder tumor.This effect may be related to

    direct cytotoxicity and immune response.It may be an effective intravesical treatment agent for superficial bladder cancer.

     Key words: Allicin; Anti tumor effect; Bladder tumor

     Liliaceae Allium garlic herb for two years, effective

    ingredient allicin, folk medicine in China has 3000 years of history. Modern research suggests that garlic has a good anti-

    tumor cancer, anti-cancer effects. Reports have suggested that garlic extract significantly inhibited the growth of bladder

    cancer, but some experimental animals died from treatment-

    related toxicity [1].

     B16 tumor cells are the University of Tennessee (Tennessee University) MSSoloway use of oral N-formamide

    (FANFT) from C3H mice induced from [2]. This poorly differentiated transitional cell carcinoma demonstrated by the transfer of properties and human transitional cell carcinoma is similar. In order to understand allicin treatment of bladder tumors and mechanism, we measured allicin in vitro growth of B16 tumor cells and to assess its in vivo anti-tumor

    effect and the impact on local immunity.

     1 Materials and methods

     1.1 Animals 30 6 ~ 8-week-old female C3H/He mice (weight

    g) from Beijing Tong Lihua Victoria Experimental Technology Co., Ltd. purchased. All experiments are in line with the guidelines for animal experiments, Guangdong Medical College.

     1.2 allicin B16 tumor cells and tumor cells by the Japanese Cancer Resource Library (JCRB) cell library provides (cell number: IFO50041). Cultured with 10% fetal calf serum

    (FBS) in DMEM (GIBCO BRL, Life Technologies, MD, USA) and 37 ? in a 5% CO2. 10000 Allicin by the Guangdong-based

    Pharmaceutical Ltd. (Approval number: State medicine accurate H44023561, production batch number 20,030,509, size 25 mg / extension; formula C6H10S3; molecular weight 178.33).

     2 Methods

     2.1 MTT experiment to establish cell culture 96-well

    plates. In each hole containing 5 × 103B16 tumor cells, each

    for a group of five holes. For the determination of allicin in vitro on tumor cell growth rate of the impact of different

    doses of allicin (2.5, 0.5,0.1,0.02 mg * ml-1) and B16 cells

    were cultured 48 h. Add MTT reagent train last 4 h. Wavelength of 560 nm on a microplate reader measured OD values.

     2.2 The animal experiments for the determination of

    allicin treatment of bladder cancer will C3H/He mice were divided into three groups, each containing 10 mice. 1 group was injected with normal saline 1 ml (control group), 2 group was injected with 12.5 mg of allicin (low-dose group), group 3

    was injected 25 mg of Allicin (high-dose group). Planted tumor

    cells were cultured with cell shovel from the bottle to collect, wash two times with normal saline. Its suspended in saline. The concentration adjusted to 1 × 106 cells / ml.

    Cultivation of tumor cells into C3H mice after the

    subcutaneous flanks, a dose of 0.1 ml. The next day will be injected into the tumor in the vicinity of allicin subcutaneous dose were 12.5 mg and 25 mg of allicin. Gauges measured twice a week with two tumor diameter to observe the

    growth of tumors [3].

     2.3 LDH release assay measuring cytotoxicity C3H/He mice were injected subcutaneously 25 mg of allicin once immunization, injected with normal saline as a negative control. Spleen cells were collected after 1 week. 96 Effect of culture medium plates with spleen cells from B16 will be target cells mixed in different proportions (mixing ratio from 12.5:1 to 100:1). Each group of three holes. Before the experiment, B16 target cells with mitomycin C injection (100 μg / ml) cultured for 1 h. 96-well culture plates in 37 ?

    CO2 incubator 24 h. By LDH cytotoxicity kit provides methods and 492 nm detection wavelength. Figure 1.

     Cytotoxic activity (%) = 100% × experimental group, LDH-

    natural release of LDH release LDH-largest natural release of