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3 'daidzein sulfonate on benign prostatic hyperplasia in mice and regulation of sex hormone balance in mice_159

By Judy Crawford,2014-10-30 08:23
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3 'daidzein sulfonate on benign prostatic hyperplasia in mice and regulation of sex hormone balance in mice_159

    3 'daidzein sulfonate on benign prostatic hyperplasia in mice and regulation of sex hormone balance in mice

     Abstract Objective To study the 3 '

     daidzein sulfonate on benign prostatic hyperplasia in mice and regulation of sex hormone balance in mice. Method of subcutaneous injection of testosterone propionate, manufacturing model of prostatic hyperplasia in mice to observe the normal control group, model group, low dose of 3'-

    daidzein sulfonate group and high-dose 3 '

     daidzein sulfonate groups of mice the prostate wet weight, prostatic index, and sex hormone effects. Results 3 '

     daidzein sulfonate on testosterone-induced prostatic

    hyperplasia has significant antagonistic effect; the same time can be significantly reduced serum T, E2, T/E2 content.

    Conclusion 3 '

     daidzein sulfonate in mice significantly inhibited prostatic hyperplasia, lower serum T, E2, T/E2 content, and thus play a role in inhibiting prostatic hyperplasia in mice.

     Key words 3 'Daidzein Sulfonate prostatic hyperplasia sex

    hormones in mice

     Abstract: ObjectiveTo study the effects of 3 '

     daidzein sulfonate sodium on Benign Prostatic Hyperplasia in mice and sexual hormone homeostasis.MethodsModels of Benign Prostatic Hyperplasia were established by hypodermic injection testosterone propionate in mice.Observe the prostate glandular wet weights, indexes of prostate glandular and sexual hormone level in control group, model group, low dose 3 '

     daidzein sulfonate sodium group and large 3'-daidzein

    sulfonate sodium group.Results3'

     daidzein sulfonate sodium apparently inhibited Benign Prostatic Hyperplasia in mice induced by testosterone propionate, obviously decreased the content of T, E2 and T/E2 in serum of mice, control the sexual hormone

    homeostasis.Conclusion3 '-daidzein sulfonate sodium can

    apparently inhibit Benign Prostatic Hyperplasia in mice induced by testosterone propionate can obviously decrease the content of serum testosterone (T) , serum estradiol (E2) and T/E2 in serum of mice, control the sexual hormone homeostasis.

     Key words: 3 '

     daidzein sulfonate sodium; Prostatic hyperplasia; Sexual hormone; Mice

     3'-daidzein sulfonate (3 '

     daidzein sulfonate sodium DSS) is the main active ingredient of Chinese medicine puerarin daidzein for structural modification and the modification of newly

    synthesized strong water-soluble substances. Traditional

    Chinese Medicine of puerarin puerarin and daidzein are more studies on pharmacological effect. We found that daidzein with anti-arrhythmia, vascular smooth muscle relaxation, inhibit cholecystokinin, hypoxia tolerance, immune enhancement and the role of local anesthesia [1 ~ 5]; It was also reported that daidzein, have antioxidant properties [6]; right EDHF induced by the long-term male rat endothelium has relaxing effects [7]; on in vitro vascular relaxation effects are [8]. However, daidzein did not dissolve in water, in the presence of clinical bioavailability of low dose disadvantages such as large and markedly slow. The 3 '

     Daidzein Daidzein Sulfonate is a structural modification and the modification of the new compounds [9], water-soluble

    strong, and is currently 3'

     Daidzein pharmacological effects of sodium only in this topic Group has reported that its anti-arrhythmic effect on

    the mouse model of experimental study of benign prostatic hyperplasia [10,11]. 3 '

     daidzein sulfonate (C15H907SNa, white crystalline powder). From Shenyang Pharmaceutical University, providing plant-based

    teaching and research, purity above 97%. Is reported that its

    benign prostatic hyperplasia in mice and regulation of sex hormone balancing effects.

     1 Materials and methods

     1.1 Drugs and reagents 3 '

     Daidzein Sulfonate: from Shenyang Pharmaceutical University, providing plant-based teaching and research.

    Diluted with saline before the experiment to the desired concentration, pH adjusted to 7.0 with NaHCO3 back. Testosterone Propionate Injection: Shanghai GM Pharmaceutical Co., Ltd. manufacture, batch number: 030,519. The forefront of

    health: Zhejiang CONBA Group Pharmaceutical Co., Ltd. manufacture, batch number: 050.65 thousand.

     1.2 Animals Adult male mice, from Jiangxi Medical College Laboratory Animal Center, Animal qualified证号:2006-075. Free

    mice in each group feeding, drinking water.

     1.3 Packet 40 adult male mice were randomly divided into five groups (n = 8), the control group, model group, the prostate group, small dose group, high-dose drug group.