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EFFECT_0

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    ;WorldJ.Acup-Maz.V.1D,No.j.March.2000 ;EFFECT0FACUPUNCTURE0NPLASMAcAMPANDcGMP ;INRATSWITHACUTECEREBRALINFARCT10N ;wu

    ;u

    ;Xuplng(

    ;.

    ;~

    ;刘叉香

    ;Zh.uShu

    ;Yi(FengYi’置言绣LjuYolIxtq?g(刘叉香)冯异)s’lnGIIe(孙国杰)HCollegeofTCM.H430061.China

    ;ABSTRACTObjective:Tostudythemechanismofacupunctureinimprovin

    gacutecerebralinfare

    ;tion.Methods:Theacutecerebr81infarctionmode1wasmadebylinearembolus.andthenthecontents

    ;ofcAMPandcGMPinplasmabeforeandafteracupunctureweredeterminedbytadioimmunoassay

    ;(RIA).Results:Atieracupuncture,theIoweredcontentofcAMPincreasedobviously.theincreased

    ;contentofcGMPdecreasedandcAMP/cGMPiose.ofthemgraduallyturnedtonormalvalue.

    ;Conclusion:AcupuncturemayimprovethebloodsupplyofthebraintissuesinthefoCUSOfinfarction

    ;byadjustingthecontentof:IPandcGMPandtheratiobetweenthem. ;acccountingfor75%[?.Resentyears’research

    ;esindicatethatthecontentsofcyclicnucleotides

    ;inplasmaandcerebrospinalfluidchangeobvious

    ;lywhendisordersofcentratnervoussystem0(2

    ;c.Thispaperissignedt0observechangesof

    ;cAMPandcGMPcontentsafteracutecerebraI

    ;infarctionandtostudytheeffectofacupuncture

    ;onthemintheratwithacutecerebralinfarction.

    ;MATERIALANDMETDs

    ;1.AnimalModel

    ;90healthySDrats.withmaleandfemale

;inequalproportions,weighingfrom260280

    ;g.wererandomlydividedintonormalgroup(n ;=10),modelgroup(n4O)andacupuncture ;thoseofthelattertwogroups

    ;furtherdividedinto4sub.

    ;groupswith10ratsineachsubgroup.Anes

    ;thetizedbyintraperitonealinjeetionof6%ehlo. ;rathydrate(0.6ml/100g),theratwasputon ;athermostatoperatingtable.Aftertheskinon ;therightpartoftheneckwasincised,theright ;conl/nol3.carotidartery(CCA),externalcarotid ;arteryanditsbrancheswereseparatedandligat

    ;ed.Thentherightinternalcarotidartery(10) ;wasseparatedwiththeproximalendbeingligat

    ;edusingasilkSUtUreandthedistalendclamped ;usinganarteryehmp.Anincisionwasmadeon ;theCCA0.5cmfromthefork.Apieceof1.5. ;gaugenylonthreadwithoneendburntintoa ;glahulartipabout025mmindiameterwasin. ;sertedintoICA.AftertheIinewentthmngh ;ICAandthebeginningofthemiddlecerebral ;Thispec

w,~,,apportedhyNit~ra|SeieaceFound~ionofHubeilaroviace(ridnttmber

    961084)

    ;

    ;WorldJ.Acup-Mox.Vo1.j0.No.1,March,200037 ;artery(MCA)totheproximalendoftheanteri

    ;orcerebralarterytoobstructallthebloodsupply ;ofMCA.thethreadwasfastened.Thesurplus ;partofthethreadwascutoff.At1asttheskin ;wasaswnup?

    ;2.MethodsofAcupuncture

    ;Accordingto”criteriaofacuOointlocations

    ;ofexperimentalanimals”workedOUtbyExperi?

    ;mentalAcupunctureandMoxibUstionInstitute ;ofChineseAssociationofAcupunctureandMox. ;ibustionin1992,Shuigou(GV26),bilateral ;Neiguan(PC6)andZusanli(ST36)wereselect? ;ed.TheneedleinsertedinShuigou(GV26)was ;obliquelyadvancedtowardsthenasalseptumby ;2mm,twirledclockwisefor9times,thenmain

    ;tainedfor20min.Theneedleinsertedin ;Neiguan(PC6)wasperpendicularlypushedon ;by23mm,whileZu~anli(ST36)by68

    ;mm.ElectroacupunctureTherapeuticInstru- ;mentG6805(madeinShanghai)wasusedto ;stimulatetheseacul~ntsfor20minwithparam

    ;eter8ofsparse-densitywaves,1mAinelectric ;currentand30Hzinfrequency.Theanimalwas ;treatedbyacupunctureasso0nasitrecovered ;fromanesthetizedstate,followedbyoncep ;day.

    ;3.PreparationofSample

    ;1.5mlbloodoftheanimalwasputintoa ;glasstubewith50ulEDTA?2Nainit.The ;tubewasshakentomakethemmixedthorongly ;andthenputintoicebathforcentrifugation

    ;(2000rpm,10rain,whichshouldbedone ;within1hour).0.1mlofthesupernatewas ;drawnouttoputintoanothertube.addedwith ;2mlabsolutealcoholandshakenfor1min.Af

    ;ter5min’svest.thenewmixturewascentrifu—

    ;gated(3o00rpm,10min).Thenewsupernate ;wasdrawnouttoputintoacleananddryPeni- ;cillinbottle.whiletheleftresiduewmixed ;th1m1of75%alcoholandsmashedbyshak

    ;ing.Andthenthemixturewascontrifugated ;(3000rpm.10min).Thesupematewasadded ;intoanotherbottle.Themixedsupematewas ;preservedin4”Cafterovendrying.

    ;4.DeterminationofSample

    ;Theradioimmunoassayreagentboxwasof

    ;feredbyShanghaiUniversityofTCM.Contents ;0{cAMPandcGMPofthebloodsampleswere ;detectedbyusingaradio-immunity-y-counter. ;Alltheprocedureswerecarriedoutfollowingthe ;directionsoftheredioimmunoassayreagentbox. ;RESULTS

    ;Thedatawerestatisticallyanalysedbya ;computerwitht-test.Theresultsareshownin ;thefollowingtable.

    ;1.EffeetofacupunctueonplasmacAMPin ;ratswithacutecerebralarction(Table1.Fig

    ;ure1).

    ;Table1.EffectofAcupunc~sreomPlmm~cAMp ;inRatswlthAcuteCerebralImt’arctloa(M?SD,p?m1)

    ;Nomad

    ;N

;ModelAcupuncture

    ;roul~K~otlp

    ;6h(10)

    ;24h(10)

    ;48h(10)

    ;72h(10)

    ;65.53?253530.11?732—36.23?694’

    ;

    ;3202?513’’4781?1302

    ;

    ;38.49?2693’5812?1614#

    ;

    ;39.98?26.186584?2455#

    ;Notes:Comparedwthnomadgroup.*P<005,*?P ;<00l;Comparedwithmodelgroup.#P<005 ;Table1andFigure1showthatthecontents ;ofplasmacAMPofmodelgroupaftinducing

    ;ischemiafor6h,24h,48h,72hwereobvionsly ;lowerthanthatofthenormalgroup(6h,24h, ;P<O.01;48h,72h,<0.05).indicatingsig- ;

    ;38WormJ.Acup-Mox.’Cot.10,No1.March.20o

;nificantdeclineofplasmacAMPafteracuteeere

    ;bralinfarction.Comparedwiththo~eofmodel ;group,thecontentofplasmacAMPofacupunc. ;turegrouprosegraduallytothenorma1levelas ;thetimewenton.Thisindicatesthatacupunc. ;turemayincreasethecontentofcAMPinplas. ;ma.

    ;Notes:noria1gr0up’?一mode1group-一一

    ;scupuncturegroup?

    ;Figure1.EffectofAcupuncture.nPlasmacAMPin ;RatswithAcuteCexehralIarction

    ;2.EffectofacupunctureonplasmacGMP ;inratswithacutecerebralinfarction(Table2, ;Figure2).

    ;cerebralischemiafor6h,24h,48h,72hwere ;highersignificantlythanthatofnormalgroup, ;indicatinganincreaseofcGMPafteracutecere. ;bralinfarction.Comparedwiththoseofmode1 ;group,thecontentsofplasmaeGMPofacupunc

    ;turegroupdecreasedgraduallynorma1levelas

    ;thetimewenton.Thisindieatesthatacupunc

    ;turemaylowerthecontentofplasmaeGMPin

    ;ratsacutecerebra1infaretion ;Notes:normslgroup一?一modelgroul~-

    ;aeup~netaregroup?

    ;Figure2EffectofAcupuncture?PhmnacGbPin

    ;RatswithAcuteCerebralInfarction ;3.EffectofacupunctureonplasmacAMP/ ;Table2.EffectofAcuplmctureORPlasmacGMPcGMPinratswithcerebrali

    nfarction(Table3,

    ;InRatslthACerebralinfarcUon(MiSD,pm/m1)Figure3).

    ;Norm

    ;N

    ;ModelAcup~cture

    ;6h(10)

    ;24h(10)

    ;48h(10)

    ;72h(10)

    ;1446?6343333?1109.29031077 ;

    ;3096?1632’23.40?1299

    ;

    ;2822?524’18.49-k307#

    ;

;2674?1085l683?586

    ;Notes;Comparedwithnormalgroup.*P<005.**P ;(001;Comparedwithmodelgroup,##F(005 ;Table3-Effect0f#.cul~mctureOilPlm-cAMP/cGMP ;inRatschAcuteCerebralInfarction(M?SD.pm/mJ) ;TaNe2andFigure2showthatcontents.f:_pa___=_P ;plasmacGMPofmodelgroupafterinducing<001;CornparedwjthTrdgr.

    ;,##P(005

    ;

    ;WorldJ.Acup-Mox.Vol10,No.1.J~darch,200039? ;Nos:nor~lsrp_?modelgroup一一一

    ;acupuncturegroup?

    ;Figure3EffectofAcupunctureonPlasmacAMP/ ;cGMPinRatSwithAcuteCerebraInfarction

    ;Table3andFigure3showthatplasma

    ;cAMP/cGMPofmodelgroupwereobviously ;lowerthanthatofnormalgroup(P<0.01) ;Comparedwiththoseofmodelgroup,cAMP/ ;cGMPofacupuncturegroupincreasedgradually ;andturnedtothenorms11eve1asthetimewent ;onThisindicatesacupuncturemayraiseplasma ;cAMP/cGMPinratswithadutecerebra1infarc

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