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EFFECT

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EFFECT

    EFFECT

;

    ;地名.

    ;40.

    ;,

    ;/

    ;幢畚

    ;WorldJAcup-Mar.Vot10.No.1,March.2000 ;鼹戆

    ;EFFECTOFELECTROACUPUNCTUREONCANINEPYL0RIC ;PRESSUREANDITSRELATIONWITH!IDE

    ;LEVELINTHEGASTRICMUCOSALTISSUES’

    ;HenngYnxin(基塑堑)SenDyong(ChuZihong(褚自宏)

    ;GaoWei(高巍)WangQingli(:P:庆莉)

    ;Dep.ofGastroenterology,Dep.ofTCM,TangduHospita1. ;theFourthMilitaryMedicalUniversity,Xi’?n710038,China

    ;ABSTRACTAimofthestudy:Toobservetheeffectofelectroacupuncture(E

    A)oncaninepyloric

    ;pressureanditsrelationwithcontentsofmotilin(MTL),somatostatin(SS)an

dnitricoxidesyn

    ;thetase(NOS)inthegastricmuoo~a1tissues.Methods:Thetota1andbasicpressureofthepyloric

    pressurewavesweremeasuredbyus;sphincter,andthefrequencyofthehigh

    ingagastrotonometer;

    ;andthecontentsofMTL,SSandNOSintissuesofthegastricbodyandgastricantrumnlucoaswere

    ;detectedbyusingtadioimmunoassay(RIA)andbiochemicalmethodsin20dogs.Results:AIterEAof

    ;Zusanli(ST36),thetotalandbasicpressureofthepyloricsphincter,andthefrequencyofthehigh.

    ;pressurewaves,thecontentofSSinthegastricbodymucoa,MTLandSSinthegastricantrummu

    ;o0saa

    decreasedsignificantly(P<0.0s)andthelevelofNOSincreasedclearly(P<0.05).While

    ;afterEAofXiajuxu(ST39),alltheindexeshadnotanystrikingchangesexceptsigmficantdecrease

    ;0fSScontentinthegastricbodymuco~t(P<0.05).Conclusions:EAhasasignificantmodulating

    ;actionongastrointestinalfunctionalactivitiesbyloweringcaninepyloricpressureandcontractedfre

;quency,whichisalsorelatedwithitsinfluenreoncontentsofsomebrain-gutpe

    ptides(BGP)andisof

    ;specificityinmeridiansandacupoints. ;KEYW0RDs

    ;,

    ;EAmodulation

    ;-r

    ;Pyloricpressurein-~utpeptid

    ;Modernclinica1observationsandexperi. ;mentalStUdiesdemonstratethatEAisoffavor. ;ablemodulatingactionongastrointestinalfunc

    ;tiona1activitiesbutitsmechanismsremainun. ;known.ItwasreportedthatEAcouldregulate ;thepressureofthepyloricsphincterJandaffect

    ;synthesisandre/easeofsomeBGPI

    ;,butits

    ;mechanismsandthepossibleconnectionbetween ;thefunctionalregulationandchangesofBGP ;levelhavenotbeenexplainedclearly.Upto ;now,anysynchronousobservationsonEA’seI—

    ;feetonpyloricpressureanditsrelationwith ;changesofsomeBGPhaveneverbeenreported

    ;injournalsbothathomeandabroad.Inthepre. ;sentstudy,weconductedsynchronousobserva

    ;tionsontheeffectofEAonthecaninePY1oric ;‘Thisstudy.subsidizedbyStateNatureScienceFoundationNo39570885

    ;

    ;WormJAcup-MoJ”.Vol0No1,March.200041

    ;pressureandtheresultantchangesofMTI,,SS ;andNOScontentsinthegastricmucosaltissues ;MATERIALSANDMETHoDS

    ;1.Materials

    ;Healthymongreldogsweighing15to20kg ;wereusedintheexperiments.Twentydogs ;wererandomlyandevenlydividedinto4groups: ;Zusanli(ST36),Xiajuxu(ST39),Nonacu ;pointandcontrolgroups.Beforeexperiments, ;theseanimalswerefastedfor24hrsbutcould ;drinkwaterfreelyOnthedayofexperiment, ;eachdogwasanesthetizedwithintravenousin

    ;jectionofpentobarbitalsodium1mg/kgAnab

    ;dominalmedianincisionwasperformedtoexpose ;thegastricbody,followedbymakingasmal1in

    ;cisionabout05cmindiameteratthefrontwalI

    ;ofthestomachbodyandinsertingaTeflon ;catheterconnectedwithapressuretransducer ;andthenwithagastrotonometer(WYY1

    ;Type,madebytheInstituteofAerospaceMedi ;calEngineering)tothepyloriccanalpartand ;fixingitThepyloricpressurewasrecorded ;2Methods

    ;BilateralZusanli(ST36)andXiajuxu(ST ;39)’]offheSt0machMeridian.ftheFoot

    ;Yangmingwereselected.Thenonmeridiannon

    ;acupointswerelocatedatthebilateralmidpoint ;betweenthesmal1headofthefihulaandthe1at ;eralmalleolus,about1cmtothelateraledgeof ;thefibula,i.e.betweenZusanli(36)and

    ;Xiajuxu(ST39)Animalsintheblankcontrol ;groupwerenotneedledandnotstimulatedelec ;tricallybuttheabovementionedindexeswere

    ;recordedordetected.A6805AElec

    ;troaeupunetureTherapeuticApparatus(madeby ;ShanghaiMedicalInstrumentFactory,)wasused ;forstimulatingtheacupointbysettingastimu

    ;latingfrequencyof50Hz.voltageof20V,m

    ;termittentwavesandadurationof30min.The ;stimulatingstrengthwasadjustedtothedegree ;whichcouldinduceslighttremorinthe1ower ;limbThepyloricpressurewasdetectedand ;recordedcontinuouslyfor2minbeforeEA,once ;every10minin90minutes’observationafter

    ;EAAtthesametime,about3gofthemucosa1 ;ti~uesfromtheslomachbodyandthegastric ;antrumwastakenandboiledinnormaIsalinefor ;5minati00.CThemucoaa1tissuesweredis

    ;sectedtobeweighted,thengroundandhomoge

    ;nizedafteradding1Naceticacid(HAc)lm1 ;Twoorthreehourslater,thehomogenatewas ;addedwiththesamevolumeof1NaOHt0be ;centrifugedat3500r/minfor15minat4.C. ;Thesupernatantwasstoredat70.Cunti1

    ;usedContentsofMTLandSSweremeasured ;byusingradioimmunoassaywithduplicatetubes ;andFJ200350GCounter(madebyStaterun

    ;262Factory)Theradioimmunoassayreagent ;kitswereprovidedbyBeijingEastAsianlm

    ;muneTechniqueInstituteandtheDepartmentof

;NeurobiologyoftheSecondMilitaryMedicalU

    ;niversity.NOScontentsweremeasuredbyusing ;biochemicalmethodandthereagentkitwassup

    ;pliedbytheRadiomedicalInstituteoftheMili

    ;taryMedicalAcademy0Dvalueswerereadand ;recordedbyusing752TypeSpectrophotometer ;3Statisticsdatawereprocessedbyem

    ;ployingNOSAsoftwareinacomputerandex ;pressedasmean=SDAvalueofP<0.05was ;acceptedasstatistically,significant. ;RESUL

    ;Changesofpyloricpressureandconcentra ;

    ;?

    ;42WorldJ.AcupMar..VdID,.No.1.March,2000

    ;tionsofMTL,SSandNOSinthemucosaltisafterEAineverygroupswerelistedinthefol

    ;suesofthegastricbodyandantrumbeforeandlowingTable. ;Table.ChangesofPylorlcPHandConcentrationsofMTL. ;SSandNOS

    ;intheGastricMuco~lTissuesbeforeandafterEAinEveryGrollps(M?SD)

    ;IndZu~rdi

;E

    ;g

    ;A

    ;ro

    ;E

    ;u

    ;A_

    ;p

    ;

    ;(n=5)

    ;Pre30rrdEAEA_PEA30minrEA.

    ;Topres- ;surefkpa) ;Basicpres- ;su(kpa) ;Frequency ;(C/~mn) ;MTLofGB ;(rig/L) ;SS0fGB

    ;(nR/L)

    ;N0SofGB

;(r~/L)

    ;MTL0fGA

    ;(L)

    ;SSofGA

    ;(ng/L)

    ;N0SGA

    ;(ng/L)

    ;287?23233?27261?33263?48269=0825.3?32282?32285?27 ;177?18132?29158?06160?69162?24156=17167?19173?38 ;480641?0347?0646?095I=0749?1346?0348?0.5 ;1472?2281563?18I164.

    ;7=2151598?1531276?2871291?3841869=2121735297 ;1695?2531282?2931434=1951028?213197I?1851773?2671497?2151

    569363

    ;4+5=0755=17451240?2747?0.349?0852?204.524 ;2094?2331560?2922351?3162108=403224+ ;7=19821622802412?2782357?313

    ;879?77718?107812?9.

    ;9869=13+I757189686?127731?212818?269 ;27?0.321?0425?0728?1032:0430?0525?0226?04 ;GB:gastricbody;GA:g~tncatrL[I;*P<0.05P<001 ;Itekinbeseenclearlythat30minafterEA

    ;ofZusanli(ST36),thetota1pressureandbasic ;pressureofthepyloricsphinctermuscle1ower ;significantly(P<005),thefrequencyofthe ;highpressurewavesdecreaseseonsiderablvfP< ;005);SScontentofthemucosa1tissuesofthe ;gastricbody,MTLandSScontentsofthegas

    ;tricantrumdecreasestrikingly(P<005)but ;NOScontentofthegastricantrumlowersappar ;ently;whileMTLandNOScontentsofthegas

    ;tricbodyhavenosignificantchangesAllthein

    ;dexesexceptSScontentinthemucosaltissuesof ;thegastricbodyinXiajuxugroupandthoseof ;Donacupointandcontrolgroupshavenotany ;considerablechanges

    ;DISCUSSIoN

    ;EAstimulationiscapableofmodulating ;functionalactivitiesofthestomach.Itstheoreti

    ;ca1basisIiesinthatthemeridiansaresubordinats ;totheinternalorgansintheinteriorandalso ;connectcloselywiththebodysurfaceincluding ;muscles,skin,etc.intheexterior.EAofRen

    ;zhong(GV26)couldsignificantlysuppressthe

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