EFFECTS

    WorldJ.Acup-Mox.Vo1.11,No.4,December,2001 ;EFFECTSOFACUPUNCTUREONTHEHIGHHEMAGGLUTINATION

    ;STATE,BLOOD.SUGAR-RAISINGHORMONEANDIMMUNOCYTE ;FACTORLEVELSINTYPE.IIDIABETESPATIENTS’

    ;ChenJianfei(谌剑飞)MaYalin(马雅玲)CaiShaohua(蔡绍华)

    ;LiangHaorong(梁浩荣)ShenJing(沈晶)

    ;TheAffiliatedZhuhaiHospitalofTCM,GuangzhouUniversityofTCM,

    ;TheKeySpecialDepartmentofNeuro—endocrineofGuangdongProvince,Zhuhai519015,China

    ;ABSTRACTObjective:Toinvestigatetheeffectofacupunctureol3highhem

    agglutinationstate,blood-sugar-raising ;hormoneandimmunocytefactorlevelsintype-IIdiabetespatients—

    ;M—

    ;e—

    ;t—

    ;ho—

    ;d—

    ;s:Atotalof120inpatientsandoutpatients

    ;wererandomlydividedintoacupunctureplusmedicationgroup(n=52)andmedicationgroup(n=5O).Inaddition,18

    ;type-IIdiabetespatientsformedacupuncturegroupforcomparingtheirtherabeuticeffects.Mainacupointsusedwere

    ;Pishu(BL20).Geshu(BL17),Yishu,Shenshu(BL23),Zusanlj(ST36),Sanyinjiao(SP6),etc..combinedwith

    ;otheracupointsaccordingtodifferentsydroms.Theseacupointswerestimulatedbymanipulaingthefiliformneedles

    ;withuniformreinforcingandreducingmethodfor15minandthenstimulatedelectricallyfor15minwithanelec-

    ;troacupuncturetherapeuticapparatus.WesternmedicinesusedwereGlipizide,DimethyldiguanideHydrochloride,

    ;etc..ThetreatmentwasgivenORcedaily,with10sessionsbeingatherapeuticcourse,2！3ccursesaltogether.1n-

    ;dexesofexternalthrombosislength(ETL),plateletagglutinationrate(PAgR),fibrinogen(FG),activatedpartial

    ;thromboplastintime(AP”1-1”),fastingbloodglucose(FBG),prothrombinti

    me(PT),adrenocoticortropichormone

    ;(ACTH),cortisol(CS),growthhormone(GH),glucagon(GL),tumornecrosisfactoralpha(TNF-a),interleukin-6

    ;(IL-6),insulin(1NS)andC-peptide(C.P)weredeterminedusingradioimmunoassay.Results:After2！3ccursesof

    ;treatment,bothacuIpuncturegroupandmedicationplusacupuncturegroupcouldsignificantlyimprovehighhemaggluti-

    ;nationstate,lowerblood-sugar-raisinghormonelevel,regulateimmunocytefactorlevelandraisethesensitivityofin-

    ;sulin,whichwereapparentlysuperiortothoseofmedicationgroup(P<0.05--0.O1)_C—

    ;oncl—

    ;usion:Acupuncturethera-

    ;PYcaneffectivelyregulateplasmablood-sugar-raisinghormone,immunocytefactorlevels,increasethesensitivityof

    ;insulintotargetcells,resistbloodcoagulationandimprovemicrocirculation. ;KEYWORDSAcupuncturetherapyType-IIdiabetesHighhemagglutinatioRstateBlood-sugar-raisinghormone

    ;Immunocytefactors

    ;Thepresentstudyisaimedtoresearchthe

    ;effectofacupunctureonhighhemagglutination

    ;state,blood-sugar-raisinghormoneandimmune—

    ;cytefactomandtoelucidateitsmechanismsin

    ;treatmentoftype.IIdiabetesfromtheview.

    ;pointsoftraditionalChinesemedicinethat”pro—

    ;metingbloodcirculationtoremovebloodstasis

    ;anddredgingmeridiancollaterals”,”strengthen—

;ingbody’sresistance,andtonilyingtheinternal

    ;organs”,andthoseofmodemneuroendocrino—

    ;logyandimmuno-network.Hereisthereport. ;1MATERIALSANDMETHoDS

    ;1.1Caseselectionandgrouping

    ;Inthisstudy,atotalof120casesofdia—

    ;beteswereinpatientsandoutpatientsfromthe ;SpecialistAmbulantClinicandIn.patientDe—

    ;partmentoftheAffiliatedZhuhaiHospitalof ;?ThisworkwassupportedbygrantfromScientificResearchFoundationofGu

    angdongProvincialAdministrationof ;TCM.

    ;

    ;WormJ.Acup-Mox.Vo1.11,No.4,December,2001 ;GuangzhouUniversityofTCM.Thesepatients ;werediagnosedastype—IIdiabetesaccordingto

    ;the”diagnosticstandardsofdiabetes”formulated

    ;bvWHODiabetesSpecialistCommitteein1985. ;Itssyndrome-differentiationandtypingwere ;carriedoutinthelightoftheclinicaltreatment ;andresearchguidingprinciplesfor”Xiaoke”(di—

    ;abetes)formulatedbyChineseHealthMin—

;istryL1j.Thosepatientswhohadsecondarydia—

    ;betes,orsevereliverinjury,kidneyinjuryorde—

    ;ficiencyofbothy/nandyangwereruledout.In ;accordancewiththerandomcardmethod,they ;wererandomlydividedintoacupunctureplus ;medicationgroup(n=52cases)andmedication ;group(n=50cases).Forcomparingthethera—

    ;peuticeffectfurther,other18caseswereat—

    ;tributedtoacupuncturegroup.Inacupuncture ;plusmedicationgroup,32weremale,20fe—

    ;male,ranginginagefrom27to66years(mean ;age=50.3?4.9years).Thedurationofdisease ;wasfromonemonthto15years(mean4.65? ;4.78years).19caseswereattributedtohyper—

    ;activityofheatduetoy/n—deficiency,33at—

    ;tributedtodeficiencyofbothqiandy/n.In ;medicationgroup,26weremaleand24female, ;ranginginagefrom28to73years(mean53.3 ;?3.5years).Thedurationofdiseasewasfrom ;onemonthto13years(mean4.38?4.55 ;years).18caseswereattributedtohyperactivity ;ofheatduetoy/n—deficiency,and32attributed

    ;todeficiencyofbothqiandy/n.A1lthepatients ;inthesetwogroupshadsimilarcomplicationsof ;vasculardisorders,peripheryneuropathyand ;bloodstasissyndromethatwerecomparablein ;clinicaldata(P>0.05).Inacupuncturegroup. ;10casesweremaleand8female,ranginginage ;from35to68years(mean45.5?11.83years). ;Thedurationofdiseasewasfrom6monthsto5 ;years(mean2.04?2.23years).8caseswere ;attributedtohyperactivityofheatduetoy/n..de.. ;ficiency,and10attributedtodeficiencyofboth ;qiand.

    ;1.2Treatmentmethods

    ;1)Acupuncturegroup:Accordingtothe ;methodsusedinthepast[！.

    ;mainacupoints

    ;includingPishu(BL20),Geshu(BL17), ;Yishu,Shenshu(BL23),Zusardi(ST36)and ;Sanyinjiao(SP6)wereemployed,supplemented ;withFeishu(BL13)andChengjiang(CV24) ;forextremethirstwithdesirefordrinksanddry—

    ;nessofmouth;withWeishu(BL21)andFeng—

    ;long(ST40)forpolyphagiawithtendencyto ;feelhungryandconstipation;withGuanyuan ;(CV4),Fuliu(KI7)andQihai(CV6)for ;polyuria,lumbarsorenessandtinnitus;andwith ;Zhongwan(CV12)andYinlingquan(SP9)for ;tiredness,diarrheaandheavinessofthelimbs. ;Theseacupointswerepuncturedwithfiliform ;needlesandstimulatedfor15minwithuniform ;reinforcingandreducingneedlingmanipulation, ;followedbyelectricalstimulation(80！120Hz,

    ;sparse-densewaves,anendurablestrengthand ;durationof15min)byusingG-6805Elec—

    ;troacupuncturePulseTherapeuticApparatus. ;Thetreatmentwasconductedoncedaily.with ;10sessionsbeingatherapeuticcourse. ;2)Medicationgroup:Patientsofmedica. ;tiongroupwereorderedtotakeGlipizide(2.5！

    ;5mg),DimethyldiguanideHydrochloride(0.25 ;！

    ;0.5g),etc.3timesdaily.

    ;3)Acupuncture+medicationgroup:The ;aforementionedmethodsofacupuncturegroup

    ;andmedicationgroupwereusedtogether.A1l ;thepatientsinthese3groupsweregivenwith2 ;！

    ;3coursesoftreatment.Duringtreatment,

    coagulation,promotingblood ;medicinesforanti—

    ;circulationandremovingbloodstasiswerenot ;used,butthepatientwasaskedtohavedietcon—

    ;tro1.

    ;1.3Indexesanddetectionmethods

    ;?Inallthepatients.indexesofadreno—

    ;coticortropichormone(ACTH),cortisol(cs), ;growthhormone(GH),glucagon(GL),tumor ;necrosisfactoralpha(TNF—a),interleukin一6

    ;

    ;?14?WorldJ.Acup-Mox.Vo1.11,No.4,December,2001 ;(IL.6),insulin(INS)andC-peptide(CP)were ;determinedusingradioimmunoassay(RIA)with ;SN.6977Counter(producedbyShanghaiHefu ;Photo-electronicInstrumentCompany)andin ;accordancewiththeinstructionsofthereagent ;kitsthatweresuppliedbyAmericanDiagnostic ;SystemsLaboratoriesInc.,BeijingNorthern

    ;Bio-teehniealInstituteandIsotopeInstituteof ;ChinaAtomicEnergyAcademy.?Indexesof

    ;externalthrombosislength(ETL),plateletag—

    ;glutinationrate(PAgR),fibrinogen(FG),acti—

    ;vatedpartialthromboplastintime(AP1Vr), ;fastingbloodglucose(FBG)andprothrombin ;time(PT)weredetectedwithRIAandthe

    ;reagentkitsweresuppliedbyScientific-technical ;CooperationInstituteofShanghaiMedicalUni—

    ;versityHuashanHospita1.?Insulinsensitivity

    ;index(ISI)wascountedaccordingtoLi’S

    ;method[引,i.e.,ISI=Ln[1/FINS(fastingin. ;sulinconcentration)×FBG(fastingblood-sugar ;concentration].FBGwasdetectedwithconven—

    ;tionalmethod.?Inlinewiththecharactersof

    ;insulinsecretion,type-IIdiabeteswasdivided ;intohighinsulinsecretion(HIS)typeandin- ;sulinsecretionrelativeinsufficiencytype ;(IIS)[.

    ;1.4Statisticalanalysis

    ;AIlthevaluesofindexeswereexpressedas ;mean?SD,theenumerationdatawereana—

    ;lyzedwithChi-squaretest,andthemeasurement ;dataanalyzedwithStudent.ttest.Thediffer. ;encevaluesbetweenpre..treatmentandpost?? ;treatmentwereexpressedasd?S.Allthedata ;wereprocessedwithMicrosoftExcel97software ;installedinapersonnelcomputer.

    ;2RESULrI’s

    ;2.1Changesofthe11indexesaftertreatment ;Aftertreatment,theseindexeswereim. ;provedindifferentdegreesinthe3groupspar—

    ;ticularlyinacupunctureplusmedicationgroup ;(Table1).

    ;Table1?Comparisonofthe3GroupsinIndexesofHighHemagg—

    ;lutinationState,

    ;Blood-siitl~lr-raisingHormonesandlmmunocyteFactor(X?S)

    ;*P<0.05.**P<0.01,***P<0.001comp~edwithpre~treatment

    ;

    ;WorldJ.Acup-Mox.Vo1.11,No.4,December,2001 ;2.2ChangesofC-P,ISIandFBGcontentsaf ;tertreatment

    ;Aftertreatment,C-P,INIandFBGcon

    ;tentschangedsignificantlyincomparisonwith