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TA cloning protocol

By Audrey Powell,2014-06-16 17:08
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For TA cloning, it is optimal if the PCR primers have G's at the 5' end as this will maximize the probability of Taq polymerase adding the terminal A overhang. If you are using a proofreading polymerase, add a bit of normal Taq to the mixture as Taq adds the terminal A's.
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