SEM I-VI - Welcome to Hemchandracharya North Gujarat University

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SEM I-VI - Welcome to Hemchandracharya North Gujarat University

    M.Sc. Microbiology CBCS Curriculum 2011

    M. Sc. (Microbiology) Syllabus

    (With effect from June 2011)

Hem. North Gujarat University, Patan Page 1

    M.Sc. Microbiology CBCS Curriculum 2011

    M.Sc. Microbiology CBCS Based Curriculum

    (With effect from June 2011)

    Paper No Title Credit

    Semester I

    MB-101 Cell Biology and Genetics Core 04 MB-102 Diversity of Life Forms and Applied Microbiology Core 04 MB-103 Biochemistry and Analytical Techniques Core 04 Elective Any one elective paper (MBEL 1 to 6) Elective 02 Practical - I Practical based on MB 101 to 103 and selected elective paper 06

    Semester II

    MB-201 Molecular Genetics of Bacteria and Bacteriophages Core 04 MB-202 Research Methodology &Professional Practices Core 04 MB-203 Bioprocess and Biochemical Engineering Core 04 Elective Any one elective paper (MBEL 7 to 12) Elective 02 Practical - II Practical based on MB 201 to 203 and selected elective paper 06

    Semester III

    MB-301 Genetic Engineering and Protein engineering Core 04 MB-302 Pharmaceutical Microbiology and Fermentation Technology Core 04 MB-303 Omics, Inter Phase Microbiology and Recent Advances in Core 04


    Elective Any one elective paper (MBEL 13 to 17) Elective 02 Practical- III Practical based on MB 301 to 303 and selected elective paper 06

    Semester IV

    MB 401 Project Work 18 MBEL 18 Any paper of students’ choice to develop technical skill Soft Skill 02

Hem. North Gujarat University, Patan Page 2

    M.Sc. Microbiology CBCS Curriculum 2011

    LIST OF ELECTIVE PAPER MB EL- 01 Advanced Bioinformatics

    MB EL-02 Microbial Genomics and Proteomics

    MB EL-03 Nanotechnology and Systems Biology

    MB EL-04 Environmentally Sound Technologies (EST) MB EL-05 Entrepreneurship and Management for Microbiology Based Industry MB EL-06 Microbiology of Extreme Environment

    MB EL-07 Quality Control , Assurance and Total Quality Management MB EL-08 Microbial Physiology and Biochemistry

    MB EL-09 Medical Microbiology and Immunology

    MB EL-10 Agriculture Microbiology

    MB EL-11 Food and Dairy Microbiology

    MB EL-12 Soil and Water Microbiology

MB EL-13 Enzyme Technology

    MB EL-14 Drug Discovery and Clinical Research

    MB EL-15 Microbial Production of Recombinant Molecules MB EL-16 Microbial Ecology

    MB EL-17 Contemporary Microbiology

MB EL-18 Soft skill

    Hem. North Gujarat University, Patan Page 3

    M.Sc. Microbiology CBCS Curriculum 2011

    Course wise distribution of contact hours, credits and examination

    scheme for CBCS system

    ; Per unit 15 hours equivalent to 1 credit per course for theory teaching. ; Total credits per course will be 4 equivalent to 60 hrs of direct theory teaching. ; For practical 2 credits per course equivalent to 4 hrs. per week, per course. (Total 6


    ; For elective paper 2 credits are allotted equivalent to 2 hrs. of counseling per week.

    (Including practical if any)

    ; For soft skill paper 2 credits are allotted equivalent to 2 hrs of counseling per week.

; Electives

    The elective course shall be offered to the students as per their choice from the list of courses provided with the curriculum. The total credits shall be 2 for each elective course including practical if any but there shall be no practical examination in the university term end examination. There shall be 2 hrs of contact teaching per week for each elective course. The question paper for end semester exam shall be of 50 marks.

; Evaluation and Examination :

    ; There shall be University examination for every core and elective course at the end

    of each semester.

    ; There shall be continuous evaluation of every student for 30% of internal

    weightage during the semester as shown below :

; Internal Assessment :

    Marks for internal assessment only for core theory papers shall be awarded on the basis of attendance/regularity/punctuality, seminars, Poster presentations, viva-voce, field

    work/library work/academic tour, MCQ’s tests, quiz competitions, assignments etc. as

    determined by the Board of Studies in the respective subject. The internal assessment marks shall be notified on the department/college notice board for information of the students and it shall be communicated to the Registrar (Evaluation) before the commencement of the University examinations.

    Any six head from the followings should be selected each of 5 marks in each theory paper for internal assessment. Following five activities are compulsory during each semester for internal continuous evaluation.

    1. Attendance

    2. Assignment /Class seminar

    3. Individual viva voce/Group discussion

    Hem. North Gujarat University, Patan Page 4

    M.Sc. Microbiology CBCS Curriculum 2011

    4. Internal MCQ based test

    5. Internal practical test/Field work

    The above five activities are to be compulsorily carried out for each semester, while out of the following three activities are to be carried out during the first three semesters respectively:

Semester I : Industrial / Educational tour

    Semester II : Seminar / Conference presentation

    Semester III: Review writing

Course contents : Each Core Course shall be divided in to FOUR units. The question paper

    for end semester examination comprises two sections of 35 marks each. One question is to be asked from each unit. There shall be equal weightage to each unit. Question paper shall consist 20% objective type question (MCQ/fill in the blanks/Answer in one word or one sentence) from all the units of the section. Each section is to be written in separate answer sheet.

    The Elective Course shall be divided into two units. The question paper for end semester examination shall be of 50 marks & will have two sections of 25 marks each. Each section of question paper will have two questions of 10 marks each. 20% objective question shall be asked in each section. Section-I of the question paper is to be drawn from unit-I and section -II is to be drawn from unit-2. Objective questions shall be asked from respective section. Moreover, there shall at least one practical test of elective paper be conducted wherever applicable. The weightage should be given in the end semester practical examination along with the core theory paper. Out of 10 marks the internal marks should be put in the end semester practical examination.

; Question Paper

    (a) Question paper shall consist of 2 sections.

    (b) Section-I covers first 2 units of the course.

    (c) Section II covers remaining 2 units of the course.

    (d) Both sections of question paper comprises two questions with internal option of 14

    marks each from each unit.

    (e) Third question shall be objective type of (07) marks which will cover both units.

    (f) Same way Section-II is to be drawn.

; Practical

     For practical, total weightage shall be 50 marks per course i.e. total of 150 marks

     for 3 core courses.

     The question paper will be as per the submitted format.

; Distribution of Marks for End Semester Examination :

    Hem. North Gujarat University, Patan Page 5

    M.Sc. Microbiology CBCS Curriculum 2011

     300 Theory Three core courses (70 + 30 =100 for each course)

     50 Theory elective course

     150 Practical (All core courses)


     Total Marks : 500

Semester IV Project Work

    Students have to take up a small research project under the supervision of a teacher from the department or may carry the work in an industry / NGO / private laboratory with required facility/other university or institution. If a student is doing project out side the university then the chemicals to carry out experiment shall be replaced to the institute where he/she carry out the experimental work. All other arrangements are to be made by the student. Student has to submit the dissertation before the last date for the submission as declared by the university.

Dissertation Thesis : 250 marks

    Presentation : 100 marks

    Related Viva : 50 marks

     Total (a) : 400 marks

General viva : 50 marks

    (all semesters)

    Regularly : _50 marks

     Total (b) : 100 marks

Grand Total (a+b) : 500 marks

    There shall be an examination for the soft skill paper at the end of the semester. The total marks for the paper shall be 50.

    Hem. North Gujarat University, Patan Page 6

    M.Sc. Microbiology CBCS Curriculum 2011

     MB - 101 Cell Biology and Genetics 4 Credits

    Section - I

    Unit 1 Cell Biology - I 15 hours

1.1. The Origin and evolution of cell, cell theory, Cell as experimental model

    1.2. Ultra-structure and function of Bacteria : Bacterial Nucleoids, Nucleosomes, Mitochondria, Microbial Cell

    Surfaces, Cytoplasmic Membranes, Capsules, Organs of Locomotion , Pili or Fimbriae 1.3. Major structural and functional features of eukaryotic cell: Cell membrane and transport across the cell

    membrane, Plasma membrane , Cell wall, Extracellular matrix and cell interaction, Nucleus, Endoplasmic

    reticulum, Golgi complex, Lysosome, Mitochondria, Chloroplast, Peroxisome, Plastids, Vacuoles, Cyto

    skeleton and Cell movement,

    1.4. Cell singling : Singling at cell surface, Singling pathway

    Unit 2 Cell Biology II 15 hours

    2.1. Cell death and Cell renewal : Programmed cell death, Stem cell and maintenance of adult tissue,

    Embryonic stem cell and therapeutic cloning

    2.2. Eukaryotic and prokaryotic cell division and cell cycle

    2.3. Host Parasite Relationship : Structures and Functions involved in HostParasite interaction,pathogenesis of

    viral diseases, pathogenesis of bacterial diseases, Microbial mechanism for escaping host defense by

    bacteria or pathogens

    2.4. Cancer : the development and cause of cancer, Tumor viruses, Onco genes, Tumor suppressor genes,

    Molecular approach to cancer treatment

    Section - II

    Unit - 3 Genetics - I 15 hours

    3.1. The organization and sequence of cellular Genomes

    3.2. Mendel‟s Principles, Concept of gene, Extensions and Modifications of Basic Principles, Sex Determination

    and Sex-Linked Characteristics

    3.3. Linkage and Mapping in Eukaryotes, Prokaryotes and Bacterial viruses

    3.4. DNA replication in prokaryotes and eukaryotes; DNA amplification and rearrangement

    Unit - 4 Genetics II 15 hours

    4.1. Gene transcription in prokaryotes and eukaryotes; post transcriptional modification 4.2. Translation in prokaryotes and eukaryotes; post translational modifications; protein processing 4.3. Gene Expression control in Eukaryotes

    4.4. Population and Evolutionary Genetics, Pedigree Analysis and Applications, Chromosome Variation

    Hem. North Gujarat University, Patan Page 7

    M.Sc. Microbiology CBCS Curriculum 2011

    List of Experiments

    1. Simple staining and Negative staining, 2. Gram‟s staining and Acid fast staining

    3. Special staining : Cell wall staining, Capsule staining, Spore staining, Flagella staining, Metachromatic

    granule staining, Nucleus staining 4. Polytene chromosome

    5. Mitosis and Meiosis

    6. Study of plant cell and animal cell 7. Demonstration of Bar body and drum stick 8. Isolation of mitochondria

    9. Isolation of chloroplast

    10. Isolation of DNA from plant animal and microbial cell

    11. Enzyme Induction

    12. Determination of G+C ratio

    List of Reference Books

1. Alexoplous, Introductory mycology

    2. Biswas, Viruses

    3. Dubey, Introduction to Fungi

    4. Flint, Virology

    5. Luria, Virology

    6. Prescott, Microbiology

    7. R. M. Atlas, Principles of Microbiology

    8. Tortora, Microbiology

    9. Perry, Microbial life

    10. Carter, Virology Principle and application

    Hem. North Gujarat University, Patan Page 8

    M.Sc. Microbiology CBCS Curriculum 2011

     MB - 102 Diversity of Life forms and Applied Microbiology 4 Credits

    Section - I

    Unit - 1 Concept of Biodiversity 15 hours

    1.1. Biodiversity Basics; Biodiversity: Origin, speciation and extinction and ecological role of biodiversity; Types

    of biodiversity: alpha, beta, and gamma diversity

    1.2. Techniques for molecular identification: Morphological methods, Biochemical, serological, Molecular

    methods, Fatty acid profiling, metabolic fingerprinting ( Biolog ), DGGE and TGGE

    1.3. Taxonomy methods: Taxonomy and classification: Taxonomic Hierarchies; Classical approach to

    classification; Numerical taxonomy; Molecular taxonomy; modern methods of taxonomy and systematic. 1.4. Overview of plant and Animal biodiversity: Taxonomic criteria of classification; Classification system;

    Distinguishing and significant characteristics of representative group

Unit - 2 Eukaryotic Microorganism and Archaebacteria 15 hours

    2.1. Mycology : Taxonomic criteria of fungal classification; Distinguishing and significant characteristics of

    Mastigomycotina, Ascomycotina, Basidiomycotina, Zygomycotina and Deuteromycotina; Fungal growth

    and differentiation;

    2.2. Protozoa: Structure, classification, reproductive strategies and economic importance; Algae : structure,

    classification, reproductive strategies and economic importance; algal ecology ; lichens 2.3. Archaebacteria : Phenotypes of Archea : Methanogenic bacteria, extremophiles : thermophilic, halophilic

    bacteria; Cell structure & composition; Eco-physiology; Taxonomy & nomenclature; Salient features of

    representative group

    2.4. Virology : Structure, classification, cultivation and economic importance of plant viruses and animal viruses;

    Structure, classification, cultivation and economic importance of Bacteriophage; Viroids; Prions

    Section II

Unit - 3 Prokaryotic Microorganismsand Viruses 15 hours

    3.1. Microbial taxonomy : Microbial evolution and diversity, Taxonomic ranks, Classification system, Major

    characteristics used in taxonomy, Assessing Microbial Phylogeny, The Major Divisions of Life, Bergey‟s

    Manual of Systematic Bacteriology, A Survey of Procaryotic Phylogeny and Diversity 3.2. Overview of the Deinococci and Nonproteobacteria Gram Negatives

    3.3. Overview Proteobacteria

    3.4. Overview of The Low G+C and High G+C Gram Positives bacteria

Unit - 4 Applied Microbiology 15 hours

4.1. Application of Microbiology and Role of Microbiologist in Dairy and Food industry

    4.2. Application of Microbiology and Role of Microbiologist in Clinical and Diagnostic field 4.3. Application of Microbiology and Role of Microbiologist in Agriculture

    4.4. Application of Microbiology and Role of Microbiologist in Environment science

    Hem. North Gujarat University, Patan Page 9

    M.Sc. Microbiology CBCS Curriculum 2011

    List of Experiments

1. Isolation and identification of bacteria belonging to Enterobacteriaceae, Bacillaceae,

    PseudomonadaceaeAzotobactereceaefamily 2. Isolation and identification of industrially important molds and fungi.

    3. Isolation of actinomyctes

    4. Isolation of bacteriophage

    5. Screening of Extremophiles (Acidophiles / Alkaliphiles / Halophiles/ Psychrophiles / Thermophiles)

    6. Study of Protozoan and algal permanent slide 7. Growth curve of E. coli and determination of growth rate and generation time, Effect of pH, temperature, salt

    and glucose on growth

    8. Identification of Microorganism with 16S rRNA homology technique

    9. Microbiological analysis of clinical sample 10. Identification of bacteria by rapid kit 11. Microbiological examination of food

    12. Microbiological examination of water 13. Microbiological analysis of Air

    14. Microbiological analysis of Milk

    List of Reference Books

1. Pollard, Cell Biology.

    2. Alberts, Molecular Biology of Cell.

    3. Coopper, The cell.

    4. Prescott, Microbiology.

    5. Lodish, Molecular Cell Biology.

    6. Griffth, An Introduction to Genetic analysis.

    7. Moat, Microbial Physiology.

    8. David White, The Physiology and Biochemistry of Prokaryotes 9. Pierce Genetics A Conceptual Approach.

    10. Benjamin, Gene VIII.

    11. Syndeer and Champness. Molecular genetics of bacteria

    12. Nancy, Fundamental Bacterial Genetics

    13. Tamarin, Principles of Genetics.

    14. J. D. Watson, Molecular Biology of the Genes.

    15. Prescott, Microbiology

    16. Griffith, Genetic Analysis

    Hem. North Gujarat University, Patan Page 10

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