By Jack Bryant,2014-05-19 09:30
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英文名称; Dimethyl Sulfoxide

    中文名称; 二甲基亚砜

    Molecular Weight 78.13344 [g/mol]

    Molecular Formula C2H6OS

    H-Bond Donor 0

    H-Bond Acceptor 1

    Formal Charge 0

    Complexity 29


    A highly polar organic liquid, that is used widely as a chemical solvent. It dissolves both polar and nonpolar compounds and is miscible in a wide range of organic solvents as well as water. Because of its ability to penetrate biological membranes, it is used as a vehicle for

     topical application of pharmaceuticals. It is also used to protect tissue during CRYOPRESERVATION. Dimethyl sulfoxide shows a range of pharmacological activity including analgesia and anti-inflammation. 生物学中的应用;

    DMSO is used in PCR to inhibit secondary structures in the DNA template or the DNA primers. It is added to the PCR mix before reacting, where it interferes with the self-complementarity of the DNA, preventing the occurrence of interfering reactions. However, use of DMSO in PCR increases the mutation rate. PCR体系中DMSO的量一般不超过总体积的4%


    In cryobiology DMSO has been used as a cryoprotectant and is still an

     important constituent of cryoprotectant vitrification mixtures used to preserve organs, tissues, and cell suspensions. Without it, up to 90 percent of frozen cells will become inactive. It is particularly important in the freezing and long-term storage of embryonic stem cells and hematopoietic stem cells, which are often frozen in a mixture

     of 10% DMSO and 90% fetal bovine serum, In the cryogenic freezine of heteroploid cell lines (MDCK, VIRO, etc)a mixture of 10% DMSO with 90% EMEM (70% EMEM + 30% fetal bovine serum + antibiotic mixture.) As

     part of an autologous bone marrow transplant the DMSO is re-infused along with the patient's own hematopoietic stem cells. 参考;

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