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tetraphenylprophrin

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tetraphenylprophrin

    Michael W. Allen

    10720 LINKWOOD CT. #1121 ? BATON ROUGE, LA 70810 ? (225) 767-3810 ? mallen@lsu.edu

    EDUCATION

     University of Kansas April 2004

     Ph.D., Bioanalytical Chemistry, with honors

     GPA: 3.7

     Bellarmine University May 1998

     Bachelor of Arts in Chemistry

     Overall GPA: 3.5 Chemistry GPA: 3.8

    PROFESSIONAL EXPERIENCE

     Postdoctoral Research Associate July 2004 May 2005

     Dr. Steven A. Soper, Advisor

     Louisiana State University

    RESEARCH EXPERIENCE Postdoctoral Research

    Dr. Steven A. Soper, advisor; Louisiana State University

    Drs. Robert P. Hammer and M. Graça H. Vicente, Louisiana State University; collaborators

    ? Collaborating with two laboratories to develop novel, near-IR fluorescent dyes for rapid, high throughput,

    multiplexed DNA analysis on microdevices

    o Explored the photophysical and spectroscopic parameters of metal-centered tetraphenylprophrin and

    phthalocyanine dyes

    o Evaluating these dyes for applications photodynamic therapy, cell organelle visualization, and single

    molecule spectroscopy

    ? Designing novel DNA primers employing near-IR dyes for multi-color, multi-lifetime analysis

    o Explored the photophysical and spectroscopic parameters of near-IR fluorescent dyes

    o Developed novel conjugation and purification protocols for the attachment of near-IR dyes Graduate Research

    Dr. Carey K. Johnson, advisor; University of Kansas

    Drs. Jeffery L. Urbauer, University of Georgia, and Thomas Squire, Pacific Northwest National Labs; collaborators

    ? Conceived, designed, and produced a sensor of protein dynamics using a fluorescently labeled protein,

    calmodulin (CaM)

    o Sensor used for multiple projects in research group and has been the basis of six publications

    o Developed HPLC methods to isolate fluorescently labeled CaM

    o Validated separation methods using mass spectrometry

    o Synthesized and purified CaM mutants and assayed their biological activity

    ? Developed a novel fluorescence assay which measures picomolar to micromolar binding affinities

    o Designed assay to examine low concentration binding affinities of drug and peptide targets of CaM ? Implemented single-molecule fluorescence experiments to investigate microsecond dynamics of CaM

    2+o Studied the kinetics of Ca binding by CaM

    o Evaluated the effects of immobilization on the activity of CaM

    ? Investigated the spectroscopy and photophysics of a fluorescent proline analogue, indoline

    Undergraduate Research

    Dr. Patrick L. Holt, advisor; Bellarmine University

    ? Developed HPLC methods for the separation fullerene (C and C) mixtures 6070

    ? Measured excited state properties by transient absorption and thermal lensing spectroscopy

     Michael Allen, Page 2 Instrumentation and Techniques

    ? HPLC (Waters, Millennium Software) ? Automated DNA synthesis ? Mass Spectrometry (ESI, TOF; MALDI) ? Confocal microscopy

    ? Fluorescence and absorption spectroscopy ? CW and ultrafast lasers (Ti:Saph, ion, dye) ? Single Molecule Spectroscopy ? Slab Gel and Capillary Electrophoresis ? Protein Expression and Purification ? Scanning Probe Microscopy. ? Affinity & Ion Exchange Chromatography

    TEACHING EXPERIENCE

    Graduate Teaching Assistantships

    ? Graduate teaching assistant for an innovative undergraduate instrumental chemistry course emphasizing

    group problem solving in the industrial chemistry setting Described in Anal. Chem. 71, 677A ? Instructed three semesters of general undergraduate chemistry laboratory sections Undergraduate Teaching Assistantships

    ? Developed a new tutoring program featuring weekly problem sessions for underclassmen chemistry courses

    ? Worked with university administrators to make permanent funded positions for three senior chemistry

    students

    AWARDS

    ? Procter & Gamble Bioanalytical Fellowship, 2003

    ? Bailey-Phillips Fellowship, University of Kansas, 1998

    ? Academic Achievement Scholarship, Bellarmine University, 1994

    AFFILIATIONS

    ? President, KU Graduate Chemistry Student Organization, 1999 2001 ? Treasurer, KU Graduate Chemistry Student Organization, 1998

    ? President, Student Section of American Chemical Society, 1997 1998 ? Director, Celebration Ringers Handbell Choir, 2001

    ? Member, American Chemical Society, 1996 - Present

    ? Member, Biophysical Society, 2000 - Present

    ? Member, Society for Applied Spectroscopy, 2003 - Present

    PUBLICATIONS

    (8) Kenneth J. Osborn, Michael W. Allen, Brian D. Slaughter, and Carey K. Johnson. Single-Molecule Fluorescence Spectroscopy: new probes of protein function and dynamics. Physiology, 2004, in press (invited review) (7) Brian D. Slaughter, Jay R. Unruh, Michael W. Allen, Ramona J. Bieber Urbauer, and Carey K. Johnson.

    Conformational Substates of Calmodulin Revealed by Single-Pair Fluorescence Resonance Energy Transfer:

    influence of solution conditions and oxidative modification. Biochemistry. 2004, submitted. (6) Michael W. Allen, Ramona J. Bieber Urbauer, and Carey K. Johnson. Single-Molecule Assays of Calmodulin Target Binding Detected with a Calmodulin Energy-Transfer Construct. Analytical Chemistry 2004, 76, 3630 - 3637. (5) Brian D. Slaughter, Michael W. Allen, Ramona J. Bieber Urbauer, and Carey K. Johnson. Single-Molecule Resonance Energy Transfer and Fluorescence Correlation Spectroscopy of Calmodulin in Solution. Journal of Physical Chemistry B 2004, 108, 10388-10397.

    (4) Michael W. Allen, Ramona J. Bieber Urbauer, Asma Zaidi, Todd D. Williams, Jeffrey L. Urbauer, and Carey K. Johnson.

    Fluorescence Labeling, Purification, and Immobilization of a Double Cysteine Mutant Calmodulin Fusion Protein for

    Single-Molecule Experiments. Analytical Biochemistry 2004, 325, 273 - 284.

    (3) Troy G. Bothwell, Michael W. Allen, John F. Hedstrom, and Carey K. Johnson. Viscosity Dependence of Side-chain and Backbone Dynamics in Peptides Containing Proline and a Fluorescent Proline Analog. Journal of Physical Chemistry B. 2004, in press.

     Michael Allen, Page 3 (2) Michael W. Allen, Jay R. Unruh, Brian D. Slaughter, Sarah J. Pyszczynski, Thaddaus R. Hellwig, Tim J. Kamerzell and

    Carey K. Johnson. The Spectroscopy and Photophysics of Indoline and Indoline-2-Carboxylic Acid. Journal of Physical Chemistry A. 2003, 107, 5660-5669.

    (1) Brian D. Slaughter, Michael W. Allen, G. H. Lushington, and Carey K. Johnson. Ab-Initio and Density Functional Study of the Electronic Transitions of Indoline and Indoline-2-Carboxylic Acid. Journal of Physical Chemistry A 2003,

    107, 5670-5680.

    Oral Presentations:

    (3) Michael W. Allen, Brian D. Slaughter, Ramona J. Bieber Urbauer, and Carey K. Johnson. Single Molecule Fluorescence Investigation of Calmodulin Function. Abstracts of Papers, 37th Midwest Regional Meeting of the

    American Chemical Society, Lawrence, KS, October 23-25, 2002.

    (2) Michael W. Allen and Patrick L. Holt. Thermal Lensing Investigation of Fullerene Photodynamics. National Conference of Undergraduate Research (NCUR), Salisbury, Maryland April 24-26, 1998. (1) Michael W. Allen, Ankit K. Chudgar, Shannon M. Weiter, and Patrick L. Holt. Thermal Lensing Investigation of Fullerene Photodynamics. Undergraduate Research Conference, Butler University, Indianapolis, IN, April 10, 1998.

    Poster Presentations:

    *(10) Michael W. Allen, Ramona J. Bieber Urbauer, and Carey K. Johnson. Probing the Micro- and Millisecond Calcium

    Dynamics of Calmodulin. Abstracts of Papers, 227th National Meeting of the American Chemical Society, Anaheim, CA,

    March 28 -April 1, 2004.

    *(9) Michael W. Allen, Brian D. Slaughter, Ramona J. Bieber Urbauer, and Carey K. Johnson. Single Molecule Immobilization and Surface Interactions of a Calcium Signaling Protein. 2004 Pittsburgh Conference on Analytical

    Chemistry and Applied Spectroscopy, PITTCON 2004. 55th Annual MeetingChicago, IL, March 7 -12, 2004.

    (8) Brian D. Slaughter*, Michael W. Allen, Ramona J. Bieber Urbauer, and Carey K. Johnson. Microsecond Time-Scale 2+Single-Molecule Dynamics of Calmodulin at Varying Ca Concentrations. Biophysical Journal, 2004, 86, 475A. Biophysical Society 48th Annual Meeting, Baltimore, MD, February 14-18, 2004.

    *(7) Michael W. Allen, Brian D. Slaughter, Ramona J. Bieber Urbauer, and Carey K. Johnson. Single Molecule Dynamics of Calmodulin-Peptide Interactions. Biophysical Journal, 2003, 84, 122A. Biophysical Society 47th Annual Meeting, San

    Antonio, TX, March 1-5, 2003.

    *(6) Carey K. Johnson, Manoj K. Singh, Kenneth D. Osborn, Michael W. Allen, Ramona J. Bieber Urbauer, and Asma Zaidi. Single-Molecule Dynamics of Target Binding by Calmodulin. Biophysical Journal, 2003, 84, 121A. Biophysical Society 47th Annual Meeting, San Antonio, TX, March 1-5, 2003.

    *(5) Brian D. Slaughter, Jay R. Unruh, Michael W. Allen, G. H. Lushington, and Carey K. Johnson. Theoretical Study of the Electronic Transitions of Indoline and Indoline-2-Carboxylic Acid and Conformations of Indoline in a Blocked

    Peptide. Biophysical Journal, 2003, 84, 347A. Biophysical Society 47th Annual Meeting, San Antonio, TX, March 1-5,

    2003.

    *(4) Michael W. Allen, Troy G. Bothwell, Brian D. Slaughter, and Carey K. Johnson. Indoline: A Novel Fluorescent Probe of Peptide Backbone Dynamics. Biophysical Journal, 2002, 82, 428A. Biophysical Society 46th Annual Meeting,

    San Francisco, CA, February 23-27, 2002.

    *(3) Kenneth D. Osborn, Ramona J. Bieber Urbauer, Michael W. Allen, and Carey K. Johnson. Single-Molecule Dynamics of Target Binding by Calmodulin. Biophysical Journal, 2002, 82, 47A. Biophysical Society 46th Annual Meeting, San

    Francisco, CA, February 23-27, 2002.

    *(2) Michael W. Allen, Troy G. Bothwell, Kenneth D. Osborn, and Carey K. Johnson. Residue Substitution Effects on Peptide Dynamics: using time-resolved fluorescence anisotropy to probe tyrosine and indoline containing peptides. Abstracts of Papers, 221st National Meeting of the American Chemical Society, San Diego, CA, April 1 - 5, 2001.

    *(1) Carey K. Johnson, Troy G. Bothwell, Michael W. Allen, and Kenneth D. Osborn. Orientational and Intramolecular Dynamics in Peptides: time-resolved fluorescence anisotropy of tyrosine and indoline fluorophores. Biophysical Journal, 2001, 80, 360a. Biophysical Society 45th Annual Meeting, Boston, MA, February 17-21, 2001.

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