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IACUC ANIMAL PROTOCOL REVIEW LIST

By Henry Richardson,2014-05-20 13:37
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2 Mar 2010Health, feed intake, and body weight will be monitored throughout the trial. Half of the pigs will be euthanized on day 21 and the remainder

    IACUC FULL COMMITTEE REVIEW SUMMARY

    March 2, 2010

    Protocol #

    10024 Impact of Floor Space Allowance on the Incidence of Morbidity and Mortality in Pigs

    ? Principal Investigator: Ellis, Michael

    ? Number and Species: 8412 Pig

    ? Review Class: B

    ? Funding: internal

    ? Primary Reviewer: Wallace

    ? Vet Reviewer: Clark

    ? Synopsis: This study will be carried out in a commercial wean-to-market site The

    Maschhoffs, located near Beardstown, Illinois. The facility consists of two buildings,

    each of which hold approximately 5,000 pigs. The buildings have fully slatted

    concrete floors and are tunnel ventilated to maintain the temperature in the building

    within the range appropriate to the size of the pig. Three floor space treatments will

    be compared: 5.0, 6.5, and 8.0 ft2/pig (0.46, 0.60, and 0.74 m2/pig, respectively).

    The floor space treatments will be created by varying the number of pigs in each pen.

    Pen dimensions are 14.4 m x 6.1 m, giving a total floor area of 87.8 m2 and group

    sizes of 185, 142, and 115 pigs (allowing for the floor space taken up by the feeders),

    respectively, to provide the required floor spaces. Two of these floor space

    treatments are below those recommended in the Ag Guide. Research performed by

    these investigators and others have suggested no impact on morbidity and mortality

    levels at floor space allowances much lower than that proposed in the Ag Guide. The

    objective of this project is to determine the relationship between floor space

    allowance and the incidence of morbidity and mortality. In order to establish this

    relationship, floor space treatments used in this study were selected to vary in equal

    amounts above and below the level that is commonly used in commercial production.

    Also, the lowest floor space is not as restrictive as it might appear. Previous floor

    space research has mainly used an average pen weight near the target market

    weight as the end point. In this study, individual pigs will be removed from the study

    pen as they reach a target live weight between 265 and 285 lb, which is typical within

    the industry. This will result in the floor space available to the remaining pigs actually

    increasing as the pigs reach the heaviest weights. The study will be carried out from

    weaning (approximately 13 lb live weight) to harvest weight (approximately 275 lb live

    weight). Pigs will be taken off test and removed from the group to be sent for harvest

    when their individual live weight is between 265 and 285 lb. There will be 19 pens of

    each floor space treatment for a total of 8,412 pigs on the study. The management

    of the pigs during the study will be in line with standard commercial practice. They

    will have ad libitum access to feed and water throughout the study period via a

    standard feeder and a cup water drinker, respectively. Diets will be formulated to

    meet or exceed the requirements proposed by the NRC (1998). All animal care and

    health issues will be dealt with by the company staff at the farm and the company

    veterinarian. UIUC research staff will have responsibility for collecting research data

    only (live weights and records of morbidity and mortality). Animals will be checked at

    least twice daily by trained personnel and any health problems will be reported to the

    company veterinarian who will decide on any treatment. Any pigs that die during the

    study or become morbid and have to be removed from the study will be weighed and

    the probable cause of the mortality (via necropsy) or morbidity recorded. At the end

    of the study period, the pigs will be harvested at a commercial packing plant and

    carcass grading data will be collected.

    ? Comments: Full committee review requested due to an Ag Guide exception for

    space requirements, but this study is designed to address morbidity, mortality, and

    IACUC Meeting List Page 1 of 4 3/2/2010

    animal care issues in commercial settings and may have a future impact on the Ag

    Guide regulations for swine housing. FCR requested by Richard Wallace. ? Stipulations: none

    Protocol #

    10027 Evaluation of Soluble Corn Fiber and Soluble Fiber Dextrin Supplementation on Indices

    of Gastrointestinal Health in the Rat

    ? Principal Investigator: Fahey, Jr., George

    ? Number and Species: 40 Rat

    ? Review Class: B

    ? Funding: internal

    ? Primary Reviewer: Roy

    ? Vet Reviewer: Criley

    ? Synopsis: Rats will be fed diets with supplementary soluble corn fiber and dextrin

    fiber, or pectin as a control or standard chow, to ascertain the effects of these dietary

    components on gut health and microflora. Four groups of rats will be fed the diets for

    21 days, then they will be euthanized, colon and cecum contents taken for analysis,

    and tissues fixed for histological evaluation. Rats will be housed in wire-bottom

    cages to prevent coprophagy and contamination of fecal matter that will be collected

    and analyzed.

    ? Comments: Full committee review requested for wire-bottom cage housing. FCR

    requested by DAR veterinarians.

    ? Stipulations: none

    Protocol #

    10028 Development of a Method for Biopsy of the Corium (Dermis) of the Hoof of Live Cattle

    and Assessing the Effect of Biopsy on Locomotion

    ? Principal Investigator: Garrett, Edgar

    ? Number and Species: 12 Cattle

    ? Review Class: B

    ? Funding: internal

    ? Primary Reviewer: Jarrell

    ? Vet Reviewer: Sauberli

    ? Synopsis: The objective of the study is to develop a procedure that can be used to

    acquire tissue samples from the corium (dermis) of the hoof of live cattle with the

    ultimate goal being to use the harvested tissue to investigate changes in histology,

    gene expression, and cytokines in order to better understand the dynamic changes in

    the hoof when exposed to different dietary or environmental conditions. This study

    seems limited to developing a method for biopsy and requests 12 cows. The lateral

    claws and lower leg (fetlock to pastern) of one front and one hind limb on opposite

    sides of the animal (e.g., left front, right rear) will be cleaned and prepared for biopsy.

    A tourniquet will be applied to each leg and the lower leg will be anesthetized with

    regional nerve blocks using lidocaine. Two holes will be drilled in each hoof and a

    biopsy punch will be used to removed a 6 mm core of laminar corium. The biopsy

    sites will be lightly bandaged and the animals will be given flunixin meglumine

    immediately after the procedure. The animals will be returned to their normal

    housing area the same day as the procedure. They will be monitored daily for seven

    days and then weekly for eight weeks for signs of lameness. The procedures,

    anesthetics, and analgesics seem appropriate as does the period of monitoring for

    the severity of the lesions created by the biopsy.

    ? Comments: The primary reviewer is not convinced that the PI needs as many

    animals as he is requesting. FCR requested by Vickie Jarrell and Debra Sauberli. ? Stipulations: The PI will be allowed to acquire tissue samples using six animals, and

    then must report back to the IACUC prior to requesting the remaining six animals (if

    still needed).

    IACUC Meeting List Page 2 of 4 3/2/2010

    Protocol #

    10032 Influence of Several Plant Extracts on Immune Response and Growth Performance of

    Weanling Pigs Challenged with PRRS Virus

    ? Principal Investigator: Pettigrew, James

    ? Number and Species: 64 Pig

    ? Review Class: B

    ? Funding: Pancosma

    ? Primary Reviewer: Morin

    ? Vet Reviewer: Allison

    ? Synopsis: The objective of this new project is to determine if feeding plant extracts

    (PE) enhances immune function and minimizes growth retardation in pigs challenged

    with PRRS virus. Piglets will be obtained from SRC at three weeks of age and

    housed in the disease-containment chambers at ERML. On arrival, pigs will be

    randomly assigned to one of eight treatment groups (eight pigs/group): (1) standard

    diet with no inoculation of PRRSV, (2) standard diet plus PE1 with no inoculation of

    PRRSV, (3) standard diet plus PE2 with no inoculation of PRRSV, (4) standard diet

    plus PE3 with no inoculation of PRRSV, (5) standard diet with inoculation of PRRSV,

    (6) standard diet plus PE1 with inoculation of PRRSV, (7) standard diet plus PE2 with

    inoculation of PRRSV, and (8) standard diet plus PE3 with inoculation of PRRSV. On

    day 14, pigs in groups 5-8 will be inoculated intranasally with live PRRS virus. Blood

    will be collected on days 14, 17, 21, and 28 for quantification of acute phase proteins

    and cytokines and isolation of mononuclear cells. Health, feed intake, and body

    weight will be monitored throughout the trial. Half of the pigs will be euthanized on

    day 21 and the remainder on day 28. Carcasses will be disposed of by incineration. ? Comments: The pens in the disease-containment chambers will provide 9 sq. ft. of

    floor space/pig. Pigs will weigh 15-17 kg by the end of the trial. The Guide

    recommends 12 sq. ft. for pigs up to 25 kg. However, the Ag Guide recommends 6

    sq. ft./pig, so the pens are compliant with Ag Guide recommendations. Pigs

    inoculated with PRRS virus are expected to exhibit fever (up to 1 C) and reduced

    body weight gain (up to 40%). If a pig develops respiratory distress or becomes

    moribund, DAR will be contacted and the pig euthanized. IBC approval for PRRS

    inoculation was on 10/5/2007. Full committee review requested because of a Guide

    exception for less than the recommended space for housing. FCR requested by

    Dawn Morin and DAR veterinarians.

    ? Stipulations: none

    Protocol #

    10034 Euthanasia of Pre-Weaned Piglets with a Novel Electrocution Device

    ? Principal Investigator: Clark, Sherrie

    ? Number and Species: 165 Pig

    ? Review Class: C

    ? Funding: internal

    ? Primary Reviewer: Novakofski

    ? Vet Reviewer: Johnson

    ? Synopsis: The purpose of this project is to test a new machine for euthanizing small

    pigs by electrocution. Currently approved methods of euthanasia by the American

    Association of Swine Veterinarians say electrocution can only be used in pigs greater

    than 10 pounds. Other methods are difficult or undesirable to use in nursery-sized

    pigs, which has led to design of a portable, enclosed device for electrocution. This

    protocol would use 165 pigs of 6 to 60 pounds to determine appropriate time of

    electrocution. Pigs will be sedated with acepromazine and ketamine prior to

    euthanasia by electrocution. Death will be determined by reflex response, ECG, and

    EEG monitoring. If short times do not result in euthanasia, pigs will be exposed to a

    second electrocution. If the second try is unsuccessful, pigs will be euthanized with

    another method. Death is the endpoint of this experiment. The need for use of

    IACUC Meeting List Page 3 of 4 3/2/2010

    target species in these tests is clear, as is the need for development of humane and

    effective methods for euthanasia.

    ? Comments: In terms of actual discomfort of the animal, the veterinary reviewer would

    categorize this protocol as Category A. Animals already selected for euthanasia will

    be used and the procedure will be conducted under anesthesia. However, since

    death is the endpoint, the protocol automatically moves to Category C. FCR

    requested by Yvette Johnson and Jan Novakofski.

    ? Stipulations: Since the animals being euthanized for this protocol will have an

    underlying health issue that makes them a candidate for euthanasia, participating in

    the protocol should not cause an undue delay in the time to euthanasia to prevent

    needless discomfort.

    IACUC Meeting List Page 4 of 4 3/2/2010

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