immediately downstream from the Renilla luciferase gene. In mutant reporter constructs, the
MRE was deleted or a three-mismatch mutation was introduced into the seed region.
The MREs of miR-125b in the 3’ UTR of human and zebrafish p53 mRNA were predicted by
TargetScan and miRBase Target
In addition, 125b-DP represses p21 expression in a dose-dependent manner, with significant
suppression still observable at a concentration as low as 10 nM of 125b-DP
The level of p53 mRNA in human lung fibroblasts, however, was not affected by the changes in
In addition, miR-125b knockdown led to a substantial increase in apoptotic cells, as quantified by
active-caspase-3 staining, while miR-125b overexpression had the opposed effect These data demonstrate/indicate/suggest/implicate/show /that
expression is inversely correlated to p53 expression spatio-temporally during development
if/whether miR-125b expression is inversely correlated to p53 expression spatio-temporally during
development, we analyzed/performe/observe /detect miR-125b expression at different stages of zebrafish embryogenesis.
The expression pattern in the brain is consistent with previously published data 明显的
prominently notably significantly; strikingly ; markedly ;Noteworthy ; marked profoundly remarkably apparent Pronounced
Interestingly, p53 and p21 expression was inversely correlated with miR-125b up-regulation over time
miR-125b expression increases steadily from 24 to 48 hpf, while p53 expression decreases
can be detected at 18 hpf and decreases to undetectable levels by 48 hp Taken together/collectively
Therefore, the p53 pathway is likely to be the major target that mediates the function
of miR-125b during the early development of zebrafish.
mRNAwas elucidated by several lines of evidence:
Our report is the first to identify a miRNA that directly regulates p53.
Modestly 适当的 This corroborates our finding that…………………. These data are consistent with a prior/previously/prior to report showing
The present study was undertaken to understand the role of…………
suggest that galectin-3 may serve as both a diagnostic marker and therapeutic target for future disease treatments.
we questioned if previous studies on galectin-3 expression in human prostate cancer using a single antibody provided the complete picture of the significance of this protein in prostate cancer.
we evaluated the role of galectin-3 during the progression of human prostate cancer using two approaches: staining human prostate cancer tissues with differential antibodies and silencing galectin-3 expression in human prostate cancer PC3 cells with siRNA.
It has been widely surmised that………………….
….have not been fully elucidated………………….