By Alfred Crawford,2014-12-31 13:57
Chlamydomonas Zeocin Transformation protocol with Glass Beads
1. Grow cells in 250ml flask with shaking in TAP medium (Fresh cells from plate
medium should be used).
2. Prepare TAP medium plates containing 10ug/ml Zeocin. (Add the Zeocin stock
solution to autoclaved medium which has been cooled to 65
3. Prepare sterile acid-washed glass bead..