By Brent Marshall,2014-03-18 22:54
Preparation and reconstitution of KvAP channels
KvAP channel protein was expressed and purified following published procedures
Following gel filtration chromatography using a Superdex-200 with 100 mM KCl, 20
mM Tris-HCl pH 8.0 and 5.0 mM decyl-maltoside (DM) the eluted channel was
concentrated to 5-10 mg/ml..