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Supplementary Methods

By Brent Marshall,2014-03-18 22:54
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1 Supplementary Methods Preparation and reconstitution of KvAP channels KvAP channel protein was expressed and purified following published procedures 1 . Following gel filtration chromatography using a Superdex-200 with 100 mM KCl, 20 mM Tris-HCl pH 8.0 and 5.0 mM decyl-maltoside (DM) the eluted channel was concentrated to 5-10 mg/ml..
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